Role of polyamines during in vitro rhizogenesis of Nothofagus nervosa using successive culture media

An in vitro rooting protocol for producing Nothofagus nervosa microplants using successive steps during tissue culture is reported. Qualitative and quantitative changes in putrescine, spermidine, and spermine tissue contents, during the rooting process and their correlation with other biochemical ma...

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Bibliographic Details
Published in:New forests 2007-07, Vol.34 (1), p.83-93
Main Authors: Martínez Pastur, G, Arena, M. E, Benavides, M. P, Eliasco, E, Curvetto, N
Format: Article
Language:English
Subjects:
Biochemical markers
Culture media
Nothofagus
Polyamine inhibitors
Polyamines
Proteins
Putrescine
rhizogenesis
Root system quality
Rooting
Rooting markers
Spermidine
Spermine
Tissue culture
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Summary:An in vitro rooting protocol for producing Nothofagus nervosa microplants using successive steps during tissue culture is reported. Qualitative and quantitative changes in putrescine, spermidine, and spermine tissue contents, during the rooting process and their correlation with other biochemical markers and morphological changes, provided the rationale for their use during the study of a successive in vitro culture protocol for N. nervosa, as well as the use of their inhibitors. The polyamine tissue content was maximum before root emergence (spermine raised to 100 nmol/g FW to later decreased to ca. 50 nmol/g FW in the following days), but it was lower in improved rooting media (197 nmol/g FW compared to 264 nmol/g FW of the control medium). It was concluded that polyamines qualitatively and quantitatively improve in vitro rhizogenesis, and the best successive culture media included 10 μM spermine during the expression phase.
ISSN:0169-4286
1573-5095
DOI:10.1007/s11056-007-9039-6