Mapping of BrdU label-retaining dental pulp cells in growing teeth and their regenerative capacity after injuries

Recent studies have demonstrated that human dental pulp contains adult stem cells. A pulse of the thymidine analog BrdU given to young animals at the optimal time could clarify where slow-cycling long-term label-retaining cells (LRCs), putative adult stem cells, reside in the pulp tissue. This study...

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Bibliographic Details
Published in:Histochemistry and cell biology 2010-09, Vol.134 (3), p.227-241
Main Authors: Ishikawa, Yuko, Ida-Yonemochi, Hiroko, Suzuki, Hironobu, Nakakura-Ohshima, Kuniko, Jung, Han-Sung, Honda, Masaki J, Ishii, Yumiko, Watanabe, Nobukazu, Ohshima, Hayato
Format: Article
Language:English
Subjects:
Adult Stem Cells - physiology
Animals
Biochemistry
Biomedical and Life Sciences
Biomedicine
Bromodeoxyuridine
Cell Biology
Cell Differentiation
Cell growth
Cell Proliferation
Dental caries
Dental Cavity Preparation
Dental Pulp - cytology
Dental Pulp - physiology
Developmental Biology
Female
Gene expression
Injuries
Mesenchymal Stromal Cells - cytology
Original Paper
Pregnancy
Rats
Rats (Wistar)
Regeneration
Rodents
Side-Population Cells - cytology
Stem cells
Teeth
Tooth Injuries - physiopathology
Tooth Replantation
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Summary:Recent studies have demonstrated that human dental pulp contains adult stem cells. A pulse of the thymidine analog BrdU given to young animals at the optimal time could clarify where slow-cycling long-term label-retaining cells (LRCs), putative adult stem cells, reside in the pulp tissue. This study focuses on the mapping of LRCs in growing teeth and their regenerative capacity after tooth injuries. Two to seven peritoneal injections of BrdU into pregnant Wistar rats revealed slow-cycling long-term dense LRCs in the mature tissues of born animals. Numerous dense LRCs were postnatally decreased in number and reached a plateau at 4 weeks after birth when they mainly resided in the center of the dental pulp, associating with blood vessels. Mature dental pulp cells were stained with Hoechst 33342 and sorted into (
ISSN:0948-6143
1432-119X
DOI:10.1007/s00418-010-0727-5