Effect of salt and osmotic stresses on germination in durum wheat (Triticum durum Desf.)

• Published in: Plant and soil 2001-04, Vol.231 (2), p.243-254
• Main Authors: Almansouri, M., Kinet, J.-M., Lutts, S.
• Format: Article
• Language: English
• Subjects: Adaptation to environment and cultivation conditions; Agronomy. Soil science and plant productions; Biological and medical sciences; Cultivars; Drought; Drought resistance; Durum wheat; Economic plant physiology; Embryo development. Germination; Embryos; Endosperm; Fundamental and applied biological sciences. Psychology; Genetics and breeding of economic plants; Germination; Growth and development; Plants; Salinity; Salts; Seedling growth; Seeds; Sodium chloride; Starches; Stress; Sugars; Table salt; Varietal selection. Specialized plant breeding, plant breeding aims; Water uptake; Wheat
• ISSN: 0032-079X; 1573-5036
• DOI: 10.1023/A:1010378409663

In order to determine the relative importance of ionic toxicity versus the osmotic component of salt stress on germination in durum wheat (Triticum durum Desf.), seeds of three cultivars differing in their salt and drought resistance (Omrabi-5, drought-resistant; Belikh, salt-resistant and Cando, salt-sensitive) were incubated in various iso-osmotic solutions of NaCl, mannitol and polyethylene-glycol (PEG) (osmotic potential of —0.15 (control solution) —0.58, —1.05 or —1.57 MPa). Moderate stress intensities only delayed germination, whereas the highest concentration of NaCl and PEG reduced final germination percentages. PEG was the most detrimental solute, while mannitol had no effect on final germination percentages. All osmotica reduced endosperm starch and soluble sugars content as well as a-amylase activities recorded after 48 h of treatment while β-amylase activities were, in contrast, slightly stimulated in all cultivars. Deleterious effects of NaCl and PEG were higher on isolated embryos germinated onto an in vitro Linsmaier and Skoog (LS) medium comparatively to whole seeds. All PEG-treated embryos, however, recovered after the stress relief while NaCl-treated embryos exhibited a lower rate of recovery and some extent of abnormal germination after rinsing. It was concluded that stress inhibition of germination could not be attributed to an inhibition of mobilisation of reserves and that the main effect of PEG occurred via an inhibition of water uptake while detrimental effects of NaCl may be linked to long-term effects of accumulated toxic ions. The behaviour of the three cultivars during germination did not fully reflect their mean level of putative stress resistance in field conditions and germination is, therefore, not recommended as a reliable selection criterion for breeding purposes.