Impact of balanced substrate flux on the metabolic process employing fuzzy logic during the cultivation of Bacillus thuringiensis var. Galleriae

The insecticidal crystal protein (ICP) synthesized at the onset of sporulation by Bacillus thuringiensis var. galleriae (Btg) is lethal against specific pests. Attempts were made to enhance the synthesis of biomass and ICP by Btg employing process optimization strategies. The process optimization wa...

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Bibliographic Details
Published in:World journal of microbiology & biotechnology 2005-03, Vol.21 (2), p.127-133
Main Authors: Anderson, R.K.I, Jayaraman, K
Format: Article
Language:English
Subjects:
Bacillus thuringiensis subsp. galleriae
Bacteria
bacterial insecticides
Biological and medical sciences
Bioreactors
biosynthesis
Biotechnology
Control systems
Cultivation
culture media
dynamic programming
Fermentation
Fluctuations
Fundamental and applied biological sciences. Psychology
Fuzzy logic
Glucose
insecticidal proteins
Optimization
process control
Yeasts
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Summary:The insecticidal crystal protein (ICP) synthesized at the onset of sporulation by Bacillus thuringiensis var. galleriae (Btg) is lethal against specific pests. Attempts were made to enhance the synthesis of biomass and ICP by Btg employing process optimization strategies. The process optimization was carried out with residual glucose concentration control in a bench scale bioreactor. A fuzzy logic-based feedback control system for maintaining the residual glucose concentration at a constant level during cultivation was developed in LabVIEW. This control system indicated the possibilities in providing a balanced substrate flux during cultivation. The identified optimum level of 2.72 g/l in residual glucose concentration was maintained by fed-batch cultivation with glucose and yeast extract fed at equal concentration with the above control system. High cell density of 16.0 g/l with specific growth rate of 0.69 h^sup -1^ was obtained during the cultivation. The balanced flux of substrate during cultivation has resulted in the enhanced synthesis of biomass and ICP. This optimized process could be commercially exploited by comparing the fluxes of basal compounds in different media sources used in fermentation.[PUBLICATION ABSTRACT]
ISSN:0959-3993
1573-0972
DOI:10.1007/s11274-004-3043-1