An Inexpensive, Relatively Green, and Rapid Method To Purify Genomic DNA from Escherichia coli: An Experiment for the Undergraduate Biochemistry Laboratory

A method to purify genomic DNA from Escherichia coli is presented. The method is an amalgam of published methods but has been modified and optimized for use in the undergraduate biochemistry laboratory. Specifically, the method uses Tide Free 2× Ultra laundry detergent, which contains unspecified pr...

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Bibliographic Details
Published in:Journal of chemical education 2010-10, Vol.87 (10), p.1113-1115
Main Authors: Sims, Paul A, Branscum, Katie M, Kao, Lydia, Keaveny, Virginia R
Format: Article
Language:English
Subjects:
Bacteria
Biochemistry
Butanol
College Science
Deoxyribonucleic acid
DNA
E coli
Ethanol
Experiments
Genetics
Genomics
Green Chemistry Laboratory
Laboratories
Laboratory Procedures
Polymerase chain reaction
Proteases
Reagents
Retail stores
Ribonuclease A
Science Instruction
Science Laboratories
Sodium
Sodium hydroxide
Undergraduate Students
Washing powders
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Summary:A method to purify genomic DNA from Escherichia coli is presented. The method is an amalgam of published methods but has been modified and optimized for use in the undergraduate biochemistry laboratory. Specifically, the method uses Tide Free 2× Ultra laundry detergent, which contains unspecified proteases and lipases, n-butanol, 2-propanol, ethanol, and ribonuclease A (or 8 mM sodium hydroxide), and requires a minimum of specialty equipment. Because a number of the required reagents are readily attainable from retail stores, the cost is low enough to allow each student to prepare a sample of purified genomic DNA, even in large laboratory classes. By following the protocol, students are able to purify genomic DNA and use this genomic DNA as a template for a polymerase chain reaction in one 3-h laboratory period.
ISSN:0021-9584
1938-1328
DOI:10.1021/ed100237e