The effect of thioacetamide on the activity and expression of cytosolic rat liver glutathione-S-transferase

The effect of thioacetamide (TA), an hepatotoxic and hepatocarcinogenic compound, on the expression and activity of the cytosolic enzyme glutathione-S-transferase (GST) was studied in rat liver. Four h following the administration of 14C-labeled thioacetamide (50 mg/Kg), several subunits of GST were...

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Bibliographic Details
Published in:Molecular and cellular biochemistry 2000-08, Vol.211 (1-2), p.103
Main Authors: Spira, B, Raw, I
Format: Article
Language:English
Subjects:
Africa, Northern
Amino Acid Sequence
Animals
Biochemistry
Carbon Radioisotopes
Chromatography, Gel
Cytosol - enzymology
Electrophoresis, Polyacrylamide Gel
Enzymes
Female
Glutathione Transferase - antagonists & inhibitors
Glutathione Transferase - genetics
Glutathione Transferase - metabolism
Liver
Liver - drug effects
Liver - enzymology
Molecular Sequence Data
Oncology
Rats
Rats, Wistar
RNA, Messenger - genetics
RNA, Messenger - metabolism
Rodents
Sequence Alignment
Thioacetamide - metabolism
Thioacetamide - pharmacology
Time Factors
Transcription, Genetic - drug effects
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Summary:The effect of thioacetamide (TA), an hepatotoxic and hepatocarcinogenic compound, on the expression and activity of the cytosolic enzyme glutathione-S-transferase (GST) was studied in rat liver. Four h following the administration of 14C-labeled thioacetamide (50 mg/Kg), several subunits of GST were found to be radioactively labeled. A single sublethal dose of TA (250 mg/Kg) decreased by three-fold the expression of class alpha GST at 24-48 h of treatment, but did not significantly affect the transcription of class mu GST. The activity of the enzyme toward 1-chloro-2,4-dinitrobenzene was mildly inhibited (66% of the control) by a 24 h TA treatment and gradually increased thereafter. It is proposed that the covalent binding of TA or its derivative to the GST subunits does not affect the activity of the enzyme. Nevertheless, GST activity inhibition is due to the deleterious effect of TA on GST transcription.
ISSN:0300-8177
1573-4919
DOI:10.1023/A:1007114801362