Morphological criteria to distinguish cell death induced by apoptotic and necrotic treatments

We present a comparative study of apoptotic and necrotic morphology (light and scanning electron microscopy), induced by well known experimental conditions (photodynamic treatments, etoposide, hydrogen peroxide, freezing-thawing and serum deprivation) on cell cultures. Our results indicate that morp...

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Published in:Apoptosis (London) 2005-01, Vol.10 (1), p.201-208
Main Authors: Rello, S, Stockert, J C, Moreno, V, Gámez, A, Pacheco, M, Juarranz, A, Cañete, M, Villanueva, A
Format: Article
Language:English
Subjects:
Apoptosis
Bubbles
Cell Death
Cellular biology
Coalescence
Comparative studies
Freezing
HeLa Cells
Humans
Hydrogen peroxide
Hydrogen Peroxide - pharmacology
Microscopy, Electron, Scanning
Necrosis - pathology
Oxidants - pharmacology
Phloroglucinol - analogs & derivatives
Phloroglucinol - pharmacology
Photosensitizing Agents - pharmacology
Porphyrins - pharmacology
Scanning electron microscopy
Thawing
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cited_by cdi_FETCH-LOGICAL-c392t-eb4311e6cd79bd1975318c536c3c5545a6dda57cbeb8eabe27ca28e395a0eb443
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container_issue 1
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container_title Apoptosis (London)
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creator Rello, S
Stockert, J C
Moreno, V
Gámez, A
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Cañete, M
Villanueva, A
description We present a comparative study of apoptotic and necrotic morphology (light and scanning electron microscopy), induced by well known experimental conditions (photodynamic treatments, etoposide, hydrogen peroxide, freezing-thawing and serum deprivation) on cell cultures. Our results indicate that morphological criteria (apoptotic cell rounding and shrinkage, and appearance of membrane bubbles in early necrosis) allow to distinguish these cell death mechanisms, and also show that, independently of the damaging agents, the necrotic process occurs in a characteristic sequence (coalescence of membrane bubbles in a single big one that detaches from cells remaining on the substrate).
doi_str_mv 10.1007/s10495-005-6075-6
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1573-675X
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subjects Apoptosis
Bubbles
Cell Death
Cellular biology
Coalescence
Comparative studies
Freezing
HeLa Cells
Humans
Hydrogen peroxide
Hydrogen Peroxide - pharmacology
Microscopy, Electron, Scanning
Necrosis - pathology
Oxidants - pharmacology
Phloroglucinol - analogs & derivatives
Phloroglucinol - pharmacology
Photosensitizing Agents - pharmacology
Porphyrins - pharmacology
Scanning electron microscopy
Thawing
title Morphological criteria to distinguish cell death induced by apoptotic and necrotic treatments
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