Fluorescence characteristics and photophysical parameters of light-harvesting chlorophyll a/b complex aggregates

Fluorescence characteristics and molecular photophysical parameters of light-harvesting chlorophyll a/b complexes isolated from pea were studied in relation to their aggregation state. The aggregate size was varied by changing the Triton X-100 concentration from 0 to 0.23 mM at a chlorophyll concent...

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Bibliographic Details
Published in:Biophysics (Oxford) 2007-10, Vol.52 (5), p.462-467
Main Authors: Ostroumov, E. E., Fadeev, V. V., Khristin, M. S., Pashchenko, V. Z., Tusov, V. B.
Format: Article
Language:English
Subjects:
Chlorophyll
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Summary:Fluorescence characteristics and molecular photophysical parameters of light-harvesting chlorophyll a/b complexes isolated from pea were studied in relation to their aggregation state. The aggregate size was varied by changing the Triton X-100 concentration from 0 to 0.23 mM at a chlorophyll concentration of 2.45 μg/ml. Molecular photophysical parameters were determined with laser fluorimetry. Dispersion of large aggregates into smaller ones drastically decreased the intensity of low-temperature (77 K) fluorescence at 700 nm, reduced the singlet-singlet annihilation rate by more than two orders of magnitude, and prolonged the fluorescence lifetime from 0.16 to 3.2 ns.[PUBLICATION ABSTRACT]
ISSN:0006-3509
1555-6654
DOI:10.1134/S0006350907050028