Antioxidant activity of free radical reactions inhibitors, applied to the dressing material for wound healing

• Published in: Biochemistry (Moscow). Supplement. Series B, Biomedical chemistry Biomedical chemistry, 2007-03, Vol.1 (1), p.45-52
• Main Authors: Klebanov, G. I., Lyubitsky, O. B., Il’ina, S. E., Medusheva, E. O., Ryltsev, V. V., Filatov, V. N.
• Format: Article
• Language: English
• Subjects: Antioxidants; Free radicals; Wound healing
• ISSN: 1990-7508; 1990-7516
• DOI: 10.1134/S1990750807010076

Antioxidant activity (AA) of inhibitors of free radical reactions (FRR) (dieton, mexidol, trypsin), applied to the dressing material for wound healing was studied using a model system containing suspension of laminated liposomes, formed from the fraction of total chicken yolk phospholipids. Lipid peroxidation (LPO) of liposome membranes was initiated by addition of Fe^sup 2+^ ions. The kinetics of FRR was monitored by coumarin enhanced chemiluminescence (CL). AA of the inhibitors was determined by their ability to intercept aqueous and hydrophobic free radicals and chelate Fe^sup 2+^ ions. Their ability to intercept radicals reduced in the following order: dieton > trypsin > mexidol. We have also found previously unknown ability of mexidol to interact with Fe^sup 2+^ and to eliminate the FRR catalyst. Studying AA of the FRR inhibitors in the two-components mixture, containing dieton and mexidol, we have observed the multifunctional effect: dieton increased the duration of latent period of CL by intersepting lipid peroxyl radicals, while mexidol, decreased this parameter by interacting with Fe^sup 2+^, i.e. mexidol masked the action of dieton. Study of AA of two-components mixture, containing mexidol and trypsin has demonstrated the same multifunctional effect. In the two-component mixture, containing trypsin and dieton, the inhibitors exhibit synergistic action. All the antioxidant properties of these FRR inhibitors were also preserved in the three component mixture. Thus, the mixture dieton, mexidol and trypsin, possesses high AA, that validates their use in the dressing materials employed for wound healing.[PUBLICATION ABSTRACT]