Structure elucidation of NeuAc, NeuGc and Kdn-containing O-glycans released from Triturus alpestris oviductal mucins: Characterization of the poly LacdiNAc sequence: HSO3(4)(GalNAc[beta]1-4GlcNAc[beta]1-3)1-3GalNAc[beta]1-4(GlcNAc[beta]1-3)0-1GlcNAc[beta]1-6GalNAc-ol

• Published in: Glycoconjugate journal 2006-07, Vol.23 (5-6), p.377
• Main Authors: Florea, Doina, Maes, Emmanuel, Guérardel, Yann, Page, Adeline, Zanetta, Jean-pierre, Cogalniceanu, Dan, Strecker, Gérard
• Format: Article
• Language: English
• Subjects: Carbohydrates; Glycoproteins; Molecular biology; Reptiles & amphibians
• ISSN: 0282-0080; 1573-4986
• DOI: 10.1007/s10719-006-6126-4

Issue Title: Special Issue: In Honor of Prof. Y. Inoue Eggs from Amphibia are surrounded by several layers of jelly that are needed for proper fertilization. Jelly coat is composed of highly glycosylated mucin-type glycoproteins containing up to 60% of carbohydrates, which display a remarkable species-specificity. This material obtained from Triturus alpestris was submitted to reductive β -elimination and the released oligosaccharide-alditols were further fractionated by HPLC. Structural characterization was performed through a combination of two dimensional ^sup 1^H-^sup 1^H and ^sup 1^H-^sup 13^C NMR and ESI-MS/MS analysis. Numerous carbohydrate chains are characterized by the presence of the Cad (Sd^sup a^) determinant, including respectively NeuAc, NeuGc or Kdn as a sialic acid. But the most significant O-glycan sequences which mark the difference between the jelly of T. alpestris and other studies amphibian jellies are polymers of GalNAc(β 1-4)GlcNAc (LacdiNAc) which form part of the following sequence: HSO^sub 3^(4)(GalNAcβ 1-4GlcNAcβ 1-3)^sub 1-3^GalNAcβ 1-4(GlcNAcβ 1-3)^sub 0-1^GlcNAcβ 1-6GalNAc-ol[PUBLICATION ABSTRACT]