Effect of nickel on ROS content and antioxidative enzyme activities in wheat leaves

• Published in: Biometals 2007-02, Vol.20 (1), p.27-36
• Main Authors: Gajewska, Ewa, Skłodowska, Maria
• Format: Article
• Language: English
• Subjects: Antioxidants; Antioxidants - metabolism; Ascorbate Peroxidases; Catalase - metabolism; Enzymatic activity; Enzymes; Enzymes - metabolism; Glutathione Peroxidase - metabolism; Hydrogen Peroxide - metabolism; Leaves; Lipid Peroxidation - drug effects; Nickel; Nickel - pharmacology; Peroxidase - metabolism; Peroxidases - metabolism; Plant Leaves - drug effects; Plant Leaves - enzymology; Plant Leaves - metabolism; Plant Proteins - metabolism; Reactive Oxygen Species - metabolism; Superoxide Dismutase - metabolism; Triticum - drug effects; Triticum - enzymology; Triticum - metabolism; Wheat
• ISSN: 0966-0844; 1572-8773
• DOI: 10.1007/s10534-006-9011-5

Influence of 100 microM Ni on growth, Ni accumulation, [Formula: see text], H2O2 and lipid peroxides contents as well as the activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), guaiacol peroxidase (POD) and glutathione peroxidase (GSH-Px) were studied in the leaves of wheat plants on the 3rd, 6th and 9th days after treatment. Exposure of the plants to Ni for only 3 days led to almost 200-fold increase in this metal concentration in the leaf tissue but later the rate of Ni accumulation was much slower. Length and fresh weight of the leaves were substantially reduced, up to 25% and 39%, respectively at the end of experiment. Visible symptoms of Ni toxicity: chlorosis and necrosis were observed following the 3rd day. Treatment with Ni resulted in the increase in [Formula: see text] and H2O2 contents in the leaves. Both showed their highest values, approximately 250% of those of the control, on the 3rd day and then their levels decreased but still markedly exceeded the control values. SOD and CAT activities decreased significantly in response to Ni treatment, however a several-fold increase in APX and POD activities was found. No significant changes in lipid peroxides content were observed in the leaves after Ni application. The activity of GSH-Px showed a 29% induction on the 3rd day. Our results indicated that despite prolonged increases in [Formula: see text] and H2O2 levels, oxidative damage, measured as the level of lipid peroxidation, did not occur in the leaves of Ni-treated wheat.