Biotin synthase ofBacillus subtilis shows less reactivity than that ofEscherichia coil in in vitro reaction systems

The biotin synthases ofBacillus subtilis andEscherichia coli were compared in a physiological reduction system using cell-free extracts and in a artificial reduction system using photo-reduced deazariboflavin. The biotin synthase ofB. subtilis was less active than that ofE. coli in both reaction sys...

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Bibliographic Details
Published in:Archives of microbiology 2002-01, Vol.179 (1), p.26-32
Main Authors: Kiyasu, Tatsuya, Asakura, Akira, Nagahashi, Yoshie, Hoshino, Tatsuo
Format: Article
Language:English
Subjects:
Biotin
Biotin synthase
Physiology
Reduction
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Summary:The biotin synthases ofBacillus subtilis andEscherichia coli were compared in a physiological reduction system using cell-free extracts and in a artificial reduction system using photo-reduced deazariboflavin. The biotin synthase ofB. subtilis was less active than that ofE. coli in both reaction systems and showed at least tenfold less biotin-forming activity than that ofE. coli in the artificial reduction system. The physiological reduction system using the biotin synthases and cell-free extracts ofB. subtilis andE. coli showed species specificity. The results suggest that the activity of the physiological reduction system ofB. subtilis is weaker than that ofE. coli. Addition of excess dethiobiotin inhibited biotin formation by growing cells ofB. subtilis, but not byE. coli.
ISSN:0302-8933
1432-072X
DOI:10.1007/s00203-002-0494-3