Identification of a Lactose-Responsive Element Upstream of the Promoter of Bacillus megaterium [beta]-Galactosidase-Encoding Gene mbgA

The Bacillus megaterium mbgA gene encodes a lactose-hydrolyzing β-galactosidase. An AraC/XylS-type activator BgaR can activate mbgA transcription in response to lactose. In this report, we show by various deletion analyses and point mutagenesis analyses that an inverted repeat centered at position -...

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Bibliographic Details
Published in:Current microbiology 2005-07, Vol.51 (1), p.31
Main Authors: Li, Jen-ming, Chiou, Chih-yung, Lee, Tian-ren, Chen, Yuan-shou, Shaw, Gwo-chyuan
Format: Article
Language:English
Subjects:
Lactose
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Summary:The Bacillus megaterium mbgA gene encodes a lactose-hydrolyzing β-galactosidase. An AraC/XylS-type activator BgaR can activate mbgA transcription in response to lactose. In this report, we show by various deletion analyses and point mutagenesis analyses that an inverted repeat centered at position -60.5 relative to the mbgA transcriptional initiation site is the cis-acting element responsible for lactose induction of mbgA expression.[PUBLICATION ABSTRACT]
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-005-4495-8