Heme oxygenase up-regulation in ultraviolet-B irradiated soybean plants involves reactive oxygen species

• Published in: Planta 2006-10, Vol.224 (5), p.1154-1162
• Main Authors: Yannarelli, Gustavo G., Noriega, Guillermo O., Batlle, Alcira, Tomaro, Maria L.
• Format: Article
• Language: English
• Subjects: Antioxidants; Ascorbate Peroxidases; Ascorbic Acid - pharmacology; Catalase - metabolism; Catalase - radiation effects; Enzymatic activity; Enzymes; Gene Expression; Glycine max - metabolism; Glycine max - radiation effects; Heme Oxygenase (Decyclizing) - metabolism; Heme Oxygenase (Decyclizing) - radiation effects; Hydrogen peroxide; Hydrogen Peroxide - pharmacology; Irradiation; Leaves; Lipid Peroxidation - radiation effects; Messenger RNA; Oxidative stress; Peroxidases - metabolism; Peroxidases - radiation effects; Plant Leaves - metabolism; Plant Leaves - radiation effects; Plants; Protein synthesis; Radiation dosage; Reactive oxygen species; Reactive Oxygen Species - metabolism; Reactive Oxygen Species - radiation effects; Soybeans; Time Factors; Transcription, Genetic - drug effects; Ultraviolet radiation; Ultraviolet Rays; Up-Regulation
• ISSN: 0032-0935; 1432-2048
• DOI: 10.1007/s00425-006-0297-x

Ultraviolet-B (UV-B) radiation has a negative impact on plant cells, and leads to the generation of reactive oxygen species (ROS). Heme oxygenase (HO, EC 1.14.99.3) plays a protective role against oxidative stress in mammals, but little is known about this issue in plants. Here, we report for the first time the response of HO in leaves of soybean (Glycine max L.) plants subjected to UV-B radiation. Under 7.5 and 15 kJ m-2 UV-B doses, HO, catalase (CAT, EC 1.11.1.6) and ascorbate peroxidase (APX, EC 1.11.1.11) activities were increased and the production of thiobarbituric acid reactive substances (TBARS) regain control values after 4 h of plant recuperation. Treatment with 30 kJ m-2 UV-B provoked a decrease in these antioxidant enzyme activities. Immunoblot analysis showed a 4.3 and 3.7-fold increase in HO-1 protein expression after irradiation with 7.5 and 15 kJ m-2, respectively. HO-1 transcript levels were enhanced (up to 77%) at these doses, as assessed by semi-quantitative RT-PCR. These data demonstrated that increased HO activity was associated with augmented protein expression and transcript levels. Plants pre-treated with the antioxidant ascorbic acid did not show the UV-B-induced up-regulation of HO-1 mRNA, but hydrogen peroxide treatment could mimic this reaction. Our results indicate that HO is up-regulated in a dose-depending manner as a mechanism of cell protection against oxidative damage and that such response occurred as a consequence of HO-1 mRNA enhancement involving ROS.