Genetic differentiation betweenBruguiera gymnorhizaandB. sexangulain Sri Lanka

The identification of populations of Bruguiera sexangula, Bruguiera gymnorhiza and their putative hybrids in the field is difficult using only morphological and phenological characters. Using a PCR based technique, RAPD (Random Amplified Polymorphic DNA), the genetic variation of Bruguiera populatio...

Full description

Saved in:
Bibliographic Details
Published in:Hydrobiologia 1999-10, Vol.413, p.11
Main Authors: Abeysinghe, Pushpa Damayanthi, Triest, Ludwig, De Greef, Bart, Koedam, Nico, Hettiarachi, Sanath
Format: Article
Language:English
Subjects:
Coastal zone
Genetic diversity
Hybrids
Morphology
Plant biology
Plant ecology
Polymorphism
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The identification of populations of Bruguiera sexangula, Bruguiera gymnorhiza and their putative hybrids in the field is difficult using only morphological and phenological characters. Using a PCR based technique, RAPD (Random Amplified Polymorphic DNA), the genetic variation of Bruguiera populations was studied from contrasting climatic and geographic regions along the southwest coastal region of Sri Lanka. Out of 45 primers screened, 20 primers allowed us to observe polymorphism, not only between species (interspecific) but also within the species (intraspecific). Analysis of RAPD data appears to be helpful in determining the genetic relationship among populations of B. gymnorhiza and B. sexangula. RAPD markers revealed that the two species are well separated without any hybrid position between the two taxa though they occur in mixed stands. Although sampling sizes of populations of this study were small, genetic variation among B. gymnorhiza and B. sexangula populations could be observed. For B. sexangula, it was possible to differentiate each of the three populations, even when using a small number of primers.[PUBLICATION ABSTRACT]
ISSN:0018-8158
1573-5117
DOI:10.1023/A:1003899028558