Immunohistochemical distribution of the tetraspanin CD9 in normal porcine tissues

The tetra-membrane-spanning protein, CD9 is a 24-27 kDa cell surface glycoprotein expressed in a wide variety of human cells being involved in a variety of cell processes, including signaling, adhesion, motility, fertilization and tumor cells metastasis. By means of a polyclonal antibody (N1) raised...

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Bibliographic Details
Published in:Molecular biology reports 2011-02, Vol.38 (2), p.1021-1028
Main Authors: Yubero, Noemí, Jiménez-Marín, Ángeles, Lucena, Concepción, Barbancho, Manuel, Garrido, Juan J
Format: Article
Language:English
Subjects:
Animal Anatomy
Animal Biochemistry
Animals
Antigens, CD - biosynthesis
Biomedical and Life Sciences
Blood Platelets - cytology
CD9
Glycoproteins
Glycoproteins - chemistry
Glycoproteins - metabolism
Histology
Hogs
Humans
Immunohistochemistry
Immunohistochemistry - methods
Leukocytes, Mononuclear - cytology
Life Sciences
Membrane Glycoproteins - biosynthesis
Molecular biology
Morphology
Polyclonal antibodies
Recombinant Proteins - chemistry
Signal Transduction
Swine
Tetraspanin
Tetraspanin-29
Tissue Distribution
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Summary:The tetra-membrane-spanning protein, CD9 is a 24-27 kDa cell surface glycoprotein expressed in a wide variety of human cells being involved in a variety of cell processes, including signaling, adhesion, motility, fertilization and tumor cells metastasis. By means of a polyclonal antibody (N1) raised against recombinant swine CD9 protein, we studied the immunohistochemical expression of CD9 on different normal swine tissues. Immunochemistry shows that swine CD9 was distribute in a similar form than in human tissues, being present on epithelial cells of lung, liver, kidney, skin, tonsil, testis (epididymo), gut mucosa, uterus and mama. Furthermore, polyclonal antibody against swine CD9 reacts with white matter from cerebrum and cerebellum, peripheral nerves fibers and Hassal corpuscle from thymus and ovum. Platelets react strongly with our antibody, but monocytes and neutrophils react lightly. These results suggest that CD9 antigen should play a similar functional role in swine and human and therefore studies on CD9 on swine as an animal model would allow new knowledge about its role in adhesion, fertilization and tumor metastasis among other important biomedical processes.
ISSN:0301-4851
1573-4978
DOI:10.1007/s11033-010-0198-8