Application of 2^sub III^^sup 7-3^ fractional factorial experimental design to enhance enzymatic activities of Pleurotus ostreatus with high concentrations of polychlorinated biphenyls

A 2... fractional factorial experimental design was used to establish 16 culture media, with and without PCBs to enhance the activities of laccase (Lac), manganese peroxidase (MnP), and versatile peroxidase (VP) produced by the white rot fungus Pleurotus ostreatus. The culture was added to 10,000 mg...

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Published in:Journal of environmental science and health. Part A, Toxic/hazardous substances & environmental engineering Toxic/hazardous substances & environmental engineering, 2011-02, Vol.46 (3), p.298
Main Authors: Gayosso-Canales, Martha, Esparza-García, Fernando J, Bermúdez-Cruz, Rosa M, Tomasini, Araceli, Ruiz-Aguilar, Graciela M L, Rodríguez-Vázquez, Refugio
Format: Article
Language:English
Subjects:
Acidity
Cell culture
Correlation analysis
Design of experiments
Enzymes
Factorial experiments
Mushrooms
PCB
Polychlorinated biphenyls
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Summary:A 2... fractional factorial experimental design was used to establish 16 culture media, with and without PCBs to enhance the activities of laccase (Lac), manganese peroxidase (MnP), and versatile peroxidase (VP) produced by the white rot fungus Pleurotus ostreatus. The culture was added to 10,000 mg L... of transformer oil, containing 71% of the identified Arochlor 1242. The culture conditions were established with eight variables at two values (levels); pH (4 and 6), agitation (100 and 200 rpm), CuSO... (150 and 250 mg L...), MnSO... (50 and 200 mg L...), Tween 80 (13 and 3500 mg L...), wheat straw (0 and 2.5 g L...), sugarcane bagasse (0 and 2.5 g L...),and Arochlor 1242 (0 and 7100 mg L...) at 4, 8, 12, 16 and 20 days old culture. Laccase activity was enhanced at a high value of pH and low value of agitation (P
ISSN:1093-4529
1532-4117