A Guided-Inquiry pH Laboratory Exercise for Introductory Biological Science Laboratories

There is a continuing need for engaging inquiry-based laboratory experiences for advanced high school and undergraduate biology courses. The authors describe a guided-inquiry exercise investigating the pH-dependence of lactase enzyme that uses an inexpensive, wide-range buffering system, lactase die...

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Bibliographic Details
Published in:Journal of college science teaching 2011-01, Vol.40 (3), p.80-89
Main Authors: Snodgrass, Meagan A., Lux, Nicholas, Metz, Anneke M.
Format: Article
Language:English
Subjects:
Biochemistry
Biology
College Science
Enzymes
Experiment design
Inquiry
Inquiry method
Introductory Courses
Laboratories
Outcomes of education
Posttests
Pretests
RESEARCH and TEACHING
Safety stock
Science activities
Science education
Science Experiments
Science Instruction
Science Laboratories
Science Process Skills
Scientific Principles
Statistical significance
Undergraduate Study
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Summary:There is a continuing need for engaging inquiry-based laboratory experiences for advanced high school and undergraduate biology courses. The authors describe a guided-inquiry exercise investigating the pH-dependence of lactase enzyme that uses an inexpensive, wide-range buffering system, lactase dietary supplement, over-the-counter glucose test strips. Learning-outcome assessments indicate the laboratory effectively teaches biology content as well as experimental assay design, and that students appreciate learning new skills as well as the challenge and rewards of designing their own experiment. (Contains 4 tables and 4 figures.)
ISSN:0047-231X
1943-4898
DOI:10.2505/3/jcst11_040_03