Alteration of [beta]-tubulin in Nicotiana plumbaginifolia confers resistance to amiprophos-methyl

A Nicotiana plumbaginifolia plant (apm5^sup r^) resistant to amiprophos-methyl (APM), a phosphoro-amide herbicide, was isolated from protoplasts prepared from leaves of haploid plants. Genetic analysis revealed that the resistance is coded for by a dominant nuclear mutation and is associated with th...

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Bibliographic Details
Published in:Theoretical and applied genetics 1998-08, Vol.97 (3), p.464
Main Authors: Blume, Y B, Strashnyuk, N M, Smertenko, A P, Solodushko, V G, Sidorov, V A, Gleba, Y Y
Format: Article
Language:English
Subjects:
Mutation
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Summary:A Nicotiana plumbaginifolia plant (apm5^sup r^) resistant to amiprophos-methyl (APM), a phosphoro-amide herbicide, was isolated from protoplasts prepared from leaves of haploid plants. Genetic analysis revealed that the resistance is coded for by a dominant nuclear mutation and is associated with the increased stability of cortical microtubules. Two-dimensional polyacrylamide-gel electrophoresis, combined with immunoblotting using anti-tubulin monoclonal antibodies, showed that part of the β-tubulin in the resistant plant possessed lower isoelectric points than the β-tubulin of susceptible wild-type plants. These results provide evidence that the resistance to APM is associated with a mutation in a β-tubulin gene. The APM-resistant line showed cross-resistance to trifluralin, a dinitroaniline herbicide, suggesting a common mechanism of resistance between these two classes of herbicides.[PUBLICATION ABSTRACT]
ISSN:0040-5752
1432-2242
DOI:10.1007/s001220050918