Diagnosis of Spontaneous Bacterial Peritonitis: Role of Tween 80 and Triton X in Ascitic Fluid Cultures

A patient with alcoholic cirrhosis of the liver, portal hypertension with hepatic encephalopathy and spontaneous bacterial peritonitis (SBP) was admitted in an obtunded condition. Attempts at delineating the aetiology of the SBP using conventional cultures as well as automated systems were not succe...

Full description

Saved in:
Bibliographic Details
Published in:Indian journal of medical microbiology 2009-04, Vol.27 (2), p.159-161
Main Authors: Iyer, R.N, Kapoor, D
Format: Article
Language:English
Subjects:
Bacteria
Blood products
Culture-negative neutrocytic ascites
Culture-negative neutrocytic ascites, triton X, tween 80
E coli
Hypertension
Immunization
Peritoneal dialysis
Respiratory system
Surfactants
triton X
tween 80
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A patient with alcoholic cirrhosis of the liver, portal hypertension with hepatic encephalopathy and spontaneous bacterial peritonitis (SBP) was admitted in an obtunded condition. Attempts at delineating the aetiology of the SBP using conventional cultures as well as automated systems were not successful. The use of non-anionic surfactant agents such as Tween 80-incorporated blood agar and Triton X treatment of the specimens facilitated the growth of Klebsiella pneumoniae from the ascitic fluid, which otherwise would have been concluded to represent culture-negative neutrocytic ascites. Thus, the use of the aforementioned agents could be explored in elucidating the aetiology of body cavity infections when conventional methods fail.
ISSN:0255-0857
1998-3646
DOI:10.4103/0255-0857.49433