Dynamics of [beta]-CH and [beta]-CH2 Groups of Amino Acid Side Chains in Proteins

• Published in: Journal of biomolecular NMR 1998-02, Vol.11 (2), p.165
• Main Authors: Engelke, Jan, Rüterjans, Heinz
• Format: Article
• Language: English
• Subjects: Amino acids; Proteins
• ISSN: 0925-2738; 1573-5001
• DOI: 10.1023/A:1008251319019

The dynamics of amino acid side chains of uniformly 13C/15N-enriched ribonuclease T1 (RNase T1) have been investigated. Heteronuclear longitudinal relaxation rates, 1H/13C NOEs, and transverse cross-correlated cross-relaxation rates between the Sx and the SxIz1Iz2 operators (SIIS cross relaxation) [Ernst and Ernst (1994) J. Magn. Reson., A110, 202-213] have been determined in this study. New pulse sequences for measuring the longitudinal relaxation time and the heteronuclear NOE of aliphatic side chain carbon nuclei were developed using the CCONH type of magnetization transfer and 1HN detection. In addition, an improved pulse sequence for the determination of the SIIS cross relaxation is presented. For the analysis of the relaxation rates, the model of restricted rotational diffusion around the χ1 dihedral angle has been applied [London and Avitabile (1978) J. Am. Chem. Soc., 100, 7159-7165]. These techniques were used in order to describe the side chain dynamics of the small globular protein RNase T1 (104 amino acids, MW about 11 kDa). Qualitative values of microdynamical parameters were obtained for 73 out of 85 amino acid side chains (glycine and alanine residues excepted) whereas more quantitative values were derived for 67 β-CH and β-CH2 groups.[PUBLICATION ABSTRACT]