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[18F]FLT PET for Non-Invasive Monitoring of Early Response to Gene Therapy in Experimental Gliomas
The purpose of this study was to investigate the potential of 3′-deoxy-3′-[ 18 F]fluorothymidine ([ 18 F]FLT) positron emission tomography (PET) to detect early treatment responses in gliomas. Human glioma cells were stably transduced with genes yielding therapeutic activity, sorted for different le...
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| Published in: | Molecular imaging and biology 2011-06, Vol.13 (3), p.547-557 |
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| container_title | Molecular imaging and biology |
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| creator | Rueger, Maria A. Ameli, Mitra Li, Hongfeng Winkeler, Alexandra Rueckriem, Benedikt Vollmar, Stefan Galldiks, Norbert Hesselmann, Volker Fraefel, Cornel Wienhard, Klaus Heiss, Wolf-Dieter Jacobs, Andreas H. |
| description | The purpose of this study was to investigate the potential of 3′-deoxy-3′-[
18
F]fluorothymidine ([
18
F]FLT) positron emission tomography (PET) to detect early treatment responses in gliomas. Human glioma cells were stably transduced with genes yielding therapeutic activity, sorted for different levels of exogenous gene expression, and implanted subcutaneously into nude mice. Multimodality imaging during prodrug therapy included (a) magnetic resonance imaging, (b) PET with 9-(4-[
18
F]fluoro-3-hydroxymethylbutyl)guanine assessing exogenous gene expression, and (c) repeat [
18
F]FLT PET assessing antiproliferative therapeutic response. All stably transduced gliomas responded to therapy with significant reduction in tumor volume and [
18
F]FLT accumulation within 3 days after initiation of therapy. The change in [
18
F]FLT uptake before and after treatment correlated to volumetrically calculated growth rates. Therapeutic efficacy as monitored by [
18
F]FLT PET correlated to levels of therapeutic gene expression measured
in vivo
. Thus, [
18
F]FLT PET assesses early antiproliferative effects, making it a promising radiotracer for the development of novel treatments for glioma. |
| doi_str_mv | 10.1007/s11307-010-0361-6 |
| format | article |
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18
F]fluorothymidine ([
18
F]FLT) positron emission tomography (PET) to detect early treatment responses in gliomas. Human glioma cells were stably transduced with genes yielding therapeutic activity, sorted for different levels of exogenous gene expression, and implanted subcutaneously into nude mice. Multimodality imaging during prodrug therapy included (a) magnetic resonance imaging, (b) PET with 9-(4-[
18
F]fluoro-3-hydroxymethylbutyl)guanine assessing exogenous gene expression, and (c) repeat [
18
F]FLT PET assessing antiproliferative therapeutic response. All stably transduced gliomas responded to therapy with significant reduction in tumor volume and [
18
F]FLT accumulation within 3 days after initiation of therapy. The change in [
18
F]FLT uptake before and after treatment correlated to volumetrically calculated growth rates. Therapeutic efficacy as monitored by [
18
F]FLT PET correlated to levels of therapeutic gene expression measured
in vivo
. Thus, [
18
F]FLT PET assesses early antiproliferative effects, making it a promising radiotracer for the development of novel treatments for glioma.</description><identifier>ISSN: 1536-1632</identifier><identifier>EISSN: 1860-2002</identifier><identifier>DOI: 10.1007/s11307-010-0361-6</identifier><identifier>PMID: 20563754</identifier><language>eng</language><publisher>New York: Springer-Verlag</publisher><subject>Animals ; Cell Line, Tumor ; Cell Proliferation ; Dideoxynucleosides ; Gene Expression ; Genetic Therapy ; Glioma - diagnostic imaging ; Glioma - pathology ; Glioma - therapy ; Green Fluorescent Proteins - metabolism ; Humans ; Imaging ; Medicine ; Medicine & Public Health ; Mice ; Mice, Nude ; Positron-Emission Tomography ; Radiology ; Research Article ; Time Factors ; Treatment Outcome ; Xenograft Model Antitumor Assays</subject><ispartof>Molecular imaging and biology, 2011-06, Vol.13 (3), p.547-557</ispartof><rights>Academy of Molecular Imaging and Society for Molecular Imaging 2010</rights><rights>Academy of Molecular Imaging and Society for Molecular Imaging 2011</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c370t-e284792c5a33097aa81aaa863425e8c06750a96b18fee6b160aaedc946f3ea3f3</citedby><cites>FETCH-LOGICAL-c370t-e284792c5a33097aa81aaa863425e8c06750a96b18fee6b160aaedc946f3ea3f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20563754$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rueger, Maria A.</creatorcontrib><creatorcontrib>Ameli, Mitra</creatorcontrib><creatorcontrib>Li, Hongfeng</creatorcontrib><creatorcontrib>Winkeler, Alexandra</creatorcontrib><creatorcontrib>Rueckriem, Benedikt</creatorcontrib><creatorcontrib>Vollmar, Stefan</creatorcontrib><creatorcontrib>Galldiks, Norbert</creatorcontrib><creatorcontrib>Hesselmann, Volker</creatorcontrib><creatorcontrib>Fraefel, Cornel</creatorcontrib><creatorcontrib>Wienhard, Klaus</creatorcontrib><creatorcontrib>Heiss, Wolf-Dieter</creatorcontrib><creatorcontrib>Jacobs, Andreas H.</creatorcontrib><title>[18F]FLT PET for Non-Invasive Monitoring of Early Response to Gene Therapy in Experimental Gliomas</title><title>Molecular imaging and biology</title><addtitle>Mol Imaging Biol</addtitle><addtitle>Mol Imaging Biol</addtitle><description>The purpose of this study was to investigate the potential of 3′-deoxy-3′-[
18
F]fluorothymidine ([
18
F]FLT) positron emission tomography (PET) to detect early treatment responses in gliomas. Human glioma cells were stably transduced with genes yielding therapeutic activity, sorted for different levels of exogenous gene expression, and implanted subcutaneously into nude mice. Multimodality imaging during prodrug therapy included (a) magnetic resonance imaging, (b) PET with 9-(4-[
18
F]fluoro-3-hydroxymethylbutyl)guanine assessing exogenous gene expression, and (c) repeat [
18
F]FLT PET assessing antiproliferative therapeutic response. All stably transduced gliomas responded to therapy with significant reduction in tumor volume and [
18
F]FLT accumulation within 3 days after initiation of therapy. The change in [
18
F]FLT uptake before and after treatment correlated to volumetrically calculated growth rates. Therapeutic efficacy as monitored by [
18
F]FLT PET correlated to levels of therapeutic gene expression measured
in vivo
. Thus, [
18
F]FLT PET assesses early antiproliferative effects, making it a promising radiotracer for the development of novel treatments for glioma.</description><subject>Animals</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation</subject><subject>Dideoxynucleosides</subject><subject>Gene Expression</subject><subject>Genetic Therapy</subject><subject>Glioma - diagnostic imaging</subject><subject>Glioma - pathology</subject><subject>Glioma - therapy</subject><subject>Green Fluorescent Proteins - metabolism</subject><subject>Humans</subject><subject>Imaging</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Mice</subject><subject>Mice, Nude</subject><subject>Positron-Emission Tomography</subject><subject>Radiology</subject><subject>Research Article</subject><subject>Time Factors</subject><subject>Treatment Outcome</subject><subject>Xenograft Model Antitumor Assays</subject><issn>1536-1632</issn><issn>1860-2002</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNp1kE9PAjEQxRujEUU_gBfTeK9O293u7tEQQBL8E4MnY5qyzOoSaNd2IfLtLQH15GXeHN57M_kRcsHhmgNkN4FzCRkDDgyk4kwdkBOeK2ACQBzGPZWKcSVFh5yGMAfgGRfymHQEpEpmaXJCpq88H7wNxhP61J_Qynn64Cwb2bUJ9RrpvbN163xt36mraN_4xYY-Y2icDUhbR4dokU4-0JtmQ2tL-18N-nqJtjULOlzUbmnCGTmqzCLg-V675GXQn_Tu2PhxOOrdjlkpM2gZijzJClGmRkooMmNybuJQMhEp5iWoLAVTqCnPK8QoCozBWVkkqpJoZCW75GrX23j3ucLQ6rlbeRtP6lwleewsIJr4zlR6F4LHSjfxX-M3moPeQtU7qDpC1VuoWsXM5b54NV3i7DfxQzEaxM4Qmi0q9H-X_2_9BmbNgH0</recordid><startdate>20110601</startdate><enddate>20110601</enddate><creator>Rueger, Maria A.</creator><creator>Ameli, Mitra</creator><creator>Li, Hongfeng</creator><creator>Winkeler, Alexandra</creator><creator>Rueckriem, Benedikt</creator><creator>Vollmar, Stefan</creator><creator>Galldiks, Norbert</creator><creator>Hesselmann, Volker</creator><creator>Fraefel, Cornel</creator><creator>Wienhard, Klaus</creator><creator>Heiss, Wolf-Dieter</creator><creator>Jacobs, Andreas H.</creator><general>Springer-Verlag</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QO</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>L6V</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope></search><sort><creationdate>20110601</creationdate><title>[18F]FLT PET for Non-Invasive Monitoring of Early Response to Gene Therapy in Experimental Gliomas</title><author>Rueger, Maria A. ; Ameli, Mitra ; Li, Hongfeng ; Winkeler, Alexandra ; Rueckriem, Benedikt ; Vollmar, Stefan ; Galldiks, Norbert ; Hesselmann, Volker ; Fraefel, Cornel ; Wienhard, Klaus ; Heiss, Wolf-Dieter ; Jacobs, Andreas H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c370t-e284792c5a33097aa81aaa863425e8c06750a96b18fee6b160aaedc946f3ea3f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Cell Line, Tumor</topic><topic>Cell Proliferation</topic><topic>Dideoxynucleosides</topic><topic>Gene Expression</topic><topic>Genetic Therapy</topic><topic>Glioma - 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18
F]fluorothymidine ([
18
F]FLT) positron emission tomography (PET) to detect early treatment responses in gliomas. Human glioma cells were stably transduced with genes yielding therapeutic activity, sorted for different levels of exogenous gene expression, and implanted subcutaneously into nude mice. Multimodality imaging during prodrug therapy included (a) magnetic resonance imaging, (b) PET with 9-(4-[
18
F]fluoro-3-hydroxymethylbutyl)guanine assessing exogenous gene expression, and (c) repeat [
18
F]FLT PET assessing antiproliferative therapeutic response. All stably transduced gliomas responded to therapy with significant reduction in tumor volume and [
18
F]FLT accumulation within 3 days after initiation of therapy. The change in [
18
F]FLT uptake before and after treatment correlated to volumetrically calculated growth rates. Therapeutic efficacy as monitored by [
18
F]FLT PET correlated to levels of therapeutic gene expression measured
in vivo
. Thus, [
18
F]FLT PET assesses early antiproliferative effects, making it a promising radiotracer for the development of novel treatments for glioma.</abstract><cop>New York</cop><pub>Springer-Verlag</pub><pmid>20563754</pmid><doi>10.1007/s11307-010-0361-6</doi><tpages>11</tpages></addata></record> |
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| language | eng |
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| subjects | Animals Cell Line, Tumor Cell Proliferation Dideoxynucleosides Gene Expression Genetic Therapy Glioma - diagnostic imaging Glioma - pathology Glioma - therapy Green Fluorescent Proteins - metabolism Humans Imaging Medicine Medicine & Public Health Mice Mice, Nude Positron-Emission Tomography Radiology Research Article Time Factors Treatment Outcome Xenograft Model Antitumor Assays |
| title | [18F]FLT PET for Non-Invasive Monitoring of Early Response to Gene Therapy in Experimental Gliomas |
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