Identification of catalytically active groups of pea (Pisum sativum L.) [beta]-glucosidase

Functional groups of cytoplasmic pea β-glucosidase pretreated to an electrophoretically homogeneous state were identified. Data on the pH dependence of the enzyme activity, calculated heat of ionization, photoinactivation of the enzyme in the presence of methylene blue, and inactivation of the enzym...

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Bibliographic Details
Published in:Applied biochemistry and microbiology 2011-05, Vol.47 (3), p.234
Main Authors: Ershova, A N, Barkalova, O N
Format: Article
Language:English
Subjects:
Biochemistry
Cytoplasm
Enzymatic activity
Enzymes
Inactivation
Ionization
Peas
Online Access:Get full text
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Summary:Functional groups of cytoplasmic pea β-glucosidase pretreated to an electrophoretically homogeneous state were identified. Data on the pH dependence of the enzyme activity, calculated heat of ionization, photoinactivation of the enzyme in the presence of methylene blue, and inactivation of the enzyme with diethyl pyrocarbonate suggest that the catalytic site of β-glucosidase contains the carboxyl group of glutamic or aspartic acids and the imidazole group of histidine.[PUBLICATION ABSTRACT]
ISSN:0003-6838
1608-3024
DOI:10.1134/S0003683811030057