Cytotoxic effects exerted by polyarylsulfone dialyser membranes depend on different sterilization processes

Polyarylsulfone group is one of the most important polymeric materials used in the biomedical field, due to its excellent properties, such as good thermal, chemical, and mechanical stability. There are three important polyarylsulfone polymers, all of which have excellent electrical properties: polys...

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Bibliographic Details
Published in:International urology and nephrology 2011-06, Vol.43 (2), p.483-490
Main Authors: Golli-Bennour, Emna El, Kouidhi, Bochra, Dey, Mouna, Younes, Rabia, Bouaziz, Chayma, Zaied, Chiraz, Bacha, Hassen, Achour, Addellatif
Format: Article
Language:English
Subjects:
Adult
Cell Survival - drug effects
Cells, Cultured
Cytotoxins
Female
Humans
Male
Medicine
Medicine & Public Health
Membranes, Artificial
Nephrology
Nephrology - Original Paper
Polymers - adverse effects
Renal Dialysis
Sterilization - methods
Sulfones - adverse effects
Urology
Young Adult
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Summary:Polyarylsulfone group is one of the most important polymeric materials used in the biomedical field, due to its excellent properties, such as good thermal, chemical, and mechanical stability. There are three important polyarylsulfone polymers, all of which have excellent electrical properties: polysulfone (PSu), polyarylsulfone (PAS) and polyarylethersulfone (PAES). All these polymers have excellent creep, radiation and high temperature resistance. In this study, we aimed to determine the effect of three sterilization processes (steam, ethylene oxide and gamma rays) on cytotoxicity of polyarylsulfone dialysis membranes. Ten long-term dialysis patients and ten age-matched healthy controls were enrolled in our study. We analysed (1) serum effect on cultured endothelial cell viability using MTT assay and (2) lipid peroxidation assessed by serum malondialdehyde (MDA) formation at the beginning (T0), the middle (T2) and the end (T4) of haemodialysis (HD) session. Our results clearly showed that steam-sterilized membranes improve endothelial cell viability when compared to ethylene oxide or gamma rays-sterilized ones. Moreover, there is a increased generation of MDA in patients sera during HD session. The serum MDA concentration was about 3, 6 and 10 times higher, respectively, for steam, ethylene oxide and gamma rays sterilization procedures when compared to the MDA amount in healthy subject sera. We concluded that using steam instead of ethylene oxide or gamma rays for sterilization may improve the biocompatibility of polyarylsulfone membranes.
ISSN:0301-1623
1573-2584
DOI:10.1007/s11255-009-9653-7