Reduction of Aflatoxin B^sub 1^ in Stored Peanuts (Arachis hypogaea L.) Using Saccharomyces cerevisiae

Aflatoxin B^sub 1^ is a toxigenic and carcinogenic compound produced by Aspergillus flavus and Aspergillus parasiticus. To inhibit aflatoxin contamination of peanuts, seeds of two peanut breeds, IAC Caiapó and IAC Runner 886, were inoculated with A. parasiticus (1.0 × 10^sup 6^ spores per ml) and th...

Full description

Saved in:
Bibliographic Details
Published in:Journal of food protection 2011-06, Vol.74 (6), p.1003
Main Authors: Prado, G, Madeira, J E G Cruz, Morais, V A D, Oliveira, M S, Souza, R A, Peluzio, J M, Godoy, I J, Silva, J F M, Pimenta, R S
Format: Article
Language:English
Subjects:
Aflatoxins
Biological control
Chromatography
Contamination
Cultivars
Fermentation
Food contamination & poisoning
Food safety
Fungi
Hydrocarbons
Legumes
Microorganisms
Pathogens
Peanuts
Pollution control
Thin layer chromatography
Yeast
Yeasts
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Aflatoxin B^sub 1^ is a toxigenic and carcinogenic compound produced by Aspergillus flavus and Aspergillus parasiticus. To inhibit aflatoxin contamination of peanuts, seeds of two peanut breeds, IAC Caiapó and IAC Runner 886, were inoculated with A. parasiticus (1.0 × 10^sup 6^ spores per ml) and the yeast Saccharomyces cerevisiae (3.2 × 10^sup 7^ cells per ml) and incubated at 25°C for 7 and 15 days. Two experiments were conducted for each incubation period separately. The treatments were completely randomized, with three replications per treatment. Treatments included the two cultivars and three types of inoculation (pathogen alone, yeast and pathogen, and yeast 3 h before pathogen). Aflatoxin B^sub 1^ was quantified with a densitometer at 366 nm after thin layer chromatography. Aflatoxin B^sub 1^ contamination in peanuts was reduced after the addition of S. cerevisiae. The concentration of aflatoxin B^sub 1^ decreased by 74.4 and 55.9% after 7 and 15 days, respectively. The greatest aflatoxin reduction was observed when S. cerevisiae was inoculated 3 h before the pathogen in IAC Caiapó seeds and incubated for 7 days at 25°C. The use of S. cerevisiae is a promising strategy for biological control of aflatoxin contamination in peanuts. [PUBLICATION ABSTRACT]
ISSN:0362-028X
1944-9097