Different effects of SNP and GSNO on mitochondrial O^sub 2^^sup .-^/H2O2 production

Sodium Nitroprusside (SNP) and S-Nitrosoglutathione (GSNO) differently affect mitochondrial H2O2 release at Complex-I. mM SNP increases while GSNO decreases the release induced by succinate alone or added on top of NAD-linked substrates. Stimulation likely depends on Nitric Oxide (^sup .^NO) (releas...

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Bibliographic Details
Published in:Journal of bioenergetics and biomembranes 2011-06, Vol.43 (3), p.267
Main Authors: Tessari, Federica, Bortolami, Silvia, Zoccarato, Franco, Alexandre, Adolfo, Cavallini, Lucia
Format: Article
Language:English
Subjects:
Bioenergetics
Cytochrome
Hydrogen peroxide
Mitochondria
Nitric oxide
Respiration
Substrates
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Summary:Sodium Nitroprusside (SNP) and S-Nitrosoglutathione (GSNO) differently affect mitochondrial H2O2 release at Complex-I. mM SNP increases while GSNO decreases the release induced by succinate alone or added on top of NAD-linked substrates. Stimulation likely depends on Nitric Oxide (^sup .^NO) (released by SNP but not by GSNO) inhibiting cytochrome oxidase and mitochondrial respiration. Preincubations with SNP or high GSNO (10 mM plus DTE to increases its ^sup .^NO release) induces an inhibition of the succinate dependent H2O2 production consistent with a ^sup .^NO dependent covalent modification. However maximal inhibition of the succinate dependent H2O2 release is obtained in the presence of low GSNO (20-100 μM), but not with SNP. This inhibition appears independent of ^sup .^NO release since μM GSNO does not affect mitochondrial respiration, or the H2O2 detection systems and its effect is very rapid. Inhibition may be partly due to an increased removal of O^sub 2^^sup .-^ since GSNO chemically competes with NBT and cytochrome C in O^sub 2^^sup .-^ detection.[PUBLICATION ABSTRACT]
ISSN:0145-479X
1573-6881
DOI:10.1007/s10863-011-9352-9