The influence of differentiation-inducing agents on plasma membrane surface characteristics of THP-1 cells

• Published in: Biochemistry (Moscow). Supplement series A, Membrane and cell biology Membrane and cell biology, 2011-06, Vol.5 (2), p.135-142
• Main Authors: Zubova, S. V., Kabanov, D. S., Ivanov, A. Yu, Voloshina, E. V., Proskuryakov, I. I., Klenina, I. B., Prokhorenko, I. R.
• Format: Article
• Language: English
• Subjects: Biochemistry; Biomedical and Life Sciences; Cell Biology; Cellular biology; Gene expression; Life Sciences; Membranes; Plasma
• ISSN: 1990-7478; 1990-7494
• DOI: 10.1134/S1990747811010120

Undifferentiated THP-1 cells from Cell Culture Collection of the Institute of Cytology, RAS (St. Petersburg), are characterized by weak expression of Toll-like receptor-4 (TLR4) on the cell surface (up to 2%) and by almost undetectable expression of CD14 and CD11b receptors. Differentiation agent phorbol-12-myristate-13-acetate independently of its concentration (2 × 10 −7 M or 10 −8 M) and incubation time (24 or 48 h) did not initiate CD11b surface expression and did not change the parameter S app (0.605 ± 0.005 at 37°C) reflecting the cell membrane viscosity. Differentiation of THP-1 cells induced by another differentiation agent, 1α,25-dihydroxyvitamin D 3 , caused expression of CD14 (up to 70–80%) and CD11b (up to 15–20%) receptors, again without changes in plasma membrane viscosity. The rate constants of the reduction of 5- and 16-doxyl-stearic acids by THP-1 cells were in the range of 6–8 × 10 −3 s −1 at 37°C. During cell differentiation significant changes in cell electrophoretic mobility (EM, μm s −1 V −1 cm) were observed. Mean value of EM for undifferentiated THP-1 cells was −1.332 ± 0.011, whereas for phorbol-12-myristate-13-acetate- and 1α,25-dihydroxyvitamin D 3 -treated cells it was −1.432 ± 0.030 and −1.212 ± 0.016, respectively.