Physiological mechanisms underlying OsNAC5-dependent tolerance of rice plants to abiotic stress

• Published in: Planta 2011-08, Vol.234 (2), p.331-345
• Main Authors: Song, Shi-Yong, Chen, Ying, Chen, Jie, Dai, Xiao-Yan, Zhang, Wen-Hao
• Format: Article
• Language: English
• Subjects: Abscisic Acid - pharmacology; Accumulation; Adaptation, Physiological - genetics; Agriculture; Arabidopsis - genetics; Arabidopsis - growth & development; Arabidopsis - physiology; Biological and medical sciences; Biomedical and Life Sciences; Carbohydrates - analysis; Cold Temperature; Drought; Droughts; Ecology; Forestry; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Plant - physiology; Genes; Germination - physiology; Hydrogen peroxide; Hydrogen Peroxide - analysis; Hydrogen Peroxide - metabolism; Ions; Life Sciences; Malondialdehyde - analysis; Malondialdehyde - metabolism; Mannitol - pharmacology; Original Article; Oryza - genetics; Oryza - growth & development; Oryza - physiology; Oxidative stress; Plant Proteins - genetics; Plant Proteins - metabolism; Plant Sciences; Plants; Plants, Genetically Modified - genetics; Plants, Genetically Modified - growth & development; Plants, Genetically Modified - physiology; Potassium - analysis; Potassium - metabolism; Proline - analysis; Proline - metabolism; Recombinant Fusion Proteins; Rice; RNA Interference; RNA, Plant - genetics; Salt tolerance; Salts; Seed germination; Seedlings; Seedlings - genetics; Seedlings - growth & development; Seedlings - physiology; Seeds - genetics; Seeds - physiology; Sodium - analysis; Sodium - metabolism; Sodium Chloride - pharmacology; Solutes; Stress, Physiological; Sugar; Sugars; Table salt; Transcription Factors - genetics; Transcription Factors - metabolism; Transgenic plants
• ISSN: 0032-0935; 1432-2048
• DOI: 10.1007/s00425-011-1403-2

To understand the functions of transcription factor OsNAC5 in response to abiotic stress, we generated transgenic rice plants with knockdown OsNAC5 by RNA-interfered (RNAi) and overexpressing OsNAC5, and investigated the effects of cold, drought and salt stress on wild-type (WT), RNAi and overexpression rice lines. Our results demonstrated that RNAi lines became less tolerant to these stresses than WT plants, while overexpression of OsNAC5 in Arabidopsis and rice enhanced tolerance to these stresses. The mechanisms underlying the changes in tolerance of the transgenic rice plants to abiotic stresses were explored by measuring free proline (Pro) and soluble sugar contents in WT and transgenic plants. Accumulation of Pro and soluble sugars was positively correlated with OsNAC5 expression levels. The less accumulation of Pro in RNAi lines may be accounted for by inhibition of Pro synthesis and transport at transcriptional levels. In addition, knockdown and overexpression of OsNAC5 enhanced and reduced accumulation of malondialdehyde and H2O2, suggesting that knockdown of OsNAC5 renders RNAi plants more susceptible to oxidative damage. The RNAi lines displayed higher Na+/K+ ratio due to greater accumulation of Na+ ions than WT under salt stress conditions, and expression of genes encoding tonoplast Na+/H+ antiporter was lower in RNAi lines than in WT under both control and salt-stressed conditions. Seed germination of RNAi and overexpression plants was more and less inhibited by salt and mannitol than that of WT, respectively. Seed germination of overexpression and RNAi plants was more and less sensitive than that of WT to ABA. These findings highlight the important role of OsNAC5 played in the tolerance of rice plants to abiotic stress by regulating downstream targets associated with accumulation of compatible solutes, Na+ ions, H2O2 and malondialdehyde.