Genome-based expression profiles as a single standardized microarray platform for the diagnosis of bladder pain syndrome/interstitial cystitis: an array of 139 genes model

• Published in: International Urogynecology Journal 2009-06, Vol.20 (6), p.743-743
• Main Authors: Tseng, Ling-Hong, Chen, Ilene, Chen, Ming-Yang, Lee, Chyi-Long, Lin, Yi-Hao, Lloyd, L. Keith
• Format: Article
• Language: English
• Subjects: Bladder; Erratum; Gene expression; Gynecology; Homeostasis; Medicine; Medicine & Public Health; Urology
• ISSN: 0937-3462; 1433-3023
• DOI: 10.1007/s00192-009-0863-4

Introduction and hypothesis The aim of the study was to investigate the molecular signatures underlying bladder pain syndrome/interstitial cystitis (BPS/IC) using cDNA microarray. Methods Microarray gene expression profiles were studied in a matched case–control study by using a system of conditional regression modeling. Results The main findings are summarized as follows: Firstly, a “139-gene” model was discovered to contain high expressions of bladder epithelium, which feature in BPS/IC. Secondly, complex metabolic reactions, including carbohydrate, lipid, cofactors, vitamins, xenobiotics, nucleotide, and amino acid metabolisms, were found to have a strong relationship with bladder smooth muscle contraction through IC status. Thirdly, we found the transcriptional regulations of IC-induced bladder smooth muscle contraction status, including the level of contractile force, tissue homeostasis, energy homeostasis, and the development of the nervous system. In addition, our study suggested the mast-cell activation mediated by the high-affinity receptor of Fc epsilon RI triggering allergic inflammation through IC status. Such genetic changes, jointly termed “bladder remodeling,” can constitute an important long-term consequence of BPS/IC. Conclusions The success of this innovation has supported the use of microarray-based expression profiling as a single standardized platform for diagnosis of BPS/IC and offers drug discovery.