The Interaction of Buccal Mucosal Epithelial Cells with E. coli Bacteria Enhances the Intraepithelial Calcium Flux and the Release of Prostaglandin E2 (PgE2)

• Published in: International Urogynecology Journal 1999-08, Vol.10 (5), p.308
• Main Authors: Mannhardt, W, Beutel, K, Habermehl, P, Knuf, M, Zepp, F
• Format: Article
• Language: English
• Subjects: Bacteria; Bacterial infections; E coli; Signal transduction
• ISSN: 0937-3462; 1433-3023
• DOI: 10.1007/s001929970007

Mucosal epithelial cells contribute significantly to host defense mechanisms. Uroepithelial cells (UEC) from healthy donors suppress bacterial growth in vitro. Bacterial adherence to UEC has been shown to be a prerequisite. Similar results have been shown for buccal epithelial cells (BEC). The host response triggered by the host-parasite interaction seems to involve signal transduction and intracellular activation of second messengers. In this study the intraepithelial calcium flux was analyzed in individual BEC after bacterial contact. BEC were derived from scrapes of the buccal mucosa and labelled with fluo-3 (a calcium indicator). Thereafter the cells were analyzed immediately with a FACscan flowcytometer. The intracellular events were evaluated before and after the addition of viable E. coli bacteria (strain 4389, K1O1H7, pili II pos.). For control, the influence of prostaglandins, histamine, PMA, LPS and opsonized avital E. coli on the epithelial calcium flux was investigated. Additionally, supernatants of BEC-E. coli cocultures were analyzed with respect to their PgE^sub 2^ content. PgE^sub 2^ concentrations in supernatants of BEC, cultured alone or together with E. coli, were measured by a commercial PgE^sub 2^ ELISA kit. The addition of vital E. coli to BEC was promptly answered by a significant intracellular calcium flux. PgE^sub 2^, histamine and PMA, but not PgF^sub 2α^, PgE^sub 1^, LPS and opsonized E. coli, increased intracellular calcium. BEC alone did not release PgE^sub 2^. After coculture with E. coli increased levels of PgE^sub 2^ were measured in the supernatants. PgE^sub 2^ release was still enhanced by coactivation of the BEC with phorbolester (PMA). Our results confirm that calcium flux in mucosal epithelial cells is stimulated by the cell-bacteria contact. We suggest that the increased PgE^sub 2^ release amplifies the stimulation of intraepithelial second messengers. The resulting cell activation may lead to the secretion of antimicrobial peptides, thereby contributing to the regulation of mucosal host resistance to bacterial infections.[PUBLICATION ABSTRACT]