Application of genetic markers to the discrimination of European Black Poplar (Populus nigra) from American Black Poplar (P. deltoides) and Hybrid Poplars (P. x canadensis) in Switzerland

European Black Poplar (Populus nigra) is considered a rare and endangered tree species because of severe reduction of its natural riverine habitat and potential hybridisation with the related non-indigenous taxa P. deltoides and P. x canadensis. As it is difficult to distinguish these taxa solely ba...

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Bibliographic Details
Published in:Trees (Berlin, West) West), 2005-11, Vol.19 (6), p.743-748
Main Authors: Holderegger, R, Angelone, S, Brodbeck, S, Csencsics, D, Gugerli, F, Hoebee, S. E, Finkeldey, R
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
allozymes
Biological and medical sciences
Deoxyribonucleic acid
DNA
Endangered plants
Endangered species
extraction
Floodplains
Fundamental and applied biological sciences. Psychology
General agronomy. Plant production
Genetic markers
hybridization
hybrids
Molecular identification
natural resource management
Plant species
Plant tissues
polymerase chain reaction
Populus canadensis
Populus deltoides
Populus nigra
Rare species
reforestation
Restoration
riparian forests
Taxa
Use of agricultural and forest wastes. Biomass use, bioconversion
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Summary:European Black Poplar (Populus nigra) is considered a rare and endangered tree species because of severe reduction of its natural riverine habitat and potential hybridisation with the related non-indigenous taxa P. deltoides and P. x canadensis. As it is difficult to distinguish these taxa solely based on their morphology, we applied a PCR-based assay with an easy-to-use and robust molecular marker set (cpDNA trnL-trnF/RsaI RFLP, nDNA win3 and nDNA POPX/MspI RFLP) in order to identify pure P. nigra. Different plant tissues could be used for fast and standardised DNA extraction. The application of the three marker types was tested on a number of different Populus taxa, and they were also used for the verification of pure P. nigra in a sample of 304 putative P. nigra individuals from Switzerland. Cross-checking of the DNA data with those using a traditional allozyme approach resulted in complete agreement. The availability of molecular identification methods is an important prerequisite for the conservation of European Black Poplar, because pure, non-introgressed plant material can then be used in restoration projects of European floodplains.
ISSN:0931-1890
1432-2285
DOI:10.1007/s00468-005-0006-6