Glutathione-mediated mineralization of 14C-labeled 2-amino-4,6-dinitrotoluene by manganese-dependent peroxidase h5 from the white-rot fungus Phanerochaete chrysosporium

Manganese-dependent peroxidase (MnP) H5 from the white-rot fungus Phanerochaete chrysosporium, in the presence of either Mn(II) (10 mM) or GSH (10 mM). was able to mineralize 14C-U-ring-labeled 2-amino-4,6-dinitrotoluene (2-A-4,6-DNT) up to 29% in 12 days. When both Mn(II) and GSH were present, the...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2000-11, Vol.54 (5), p.659-664
Main Authors: VAN AKEN, B, CAMERON, M. D, STAHL, J. D, PLUMAT, A, NAVEAU, H, AUST, S. D, AGATHOS, S. N
Format: Article
Language:English
Subjects:
Aniline Compounds - metabolism
Bioconversions. Hemisynthesis
Biodegradation, Environmental
Biological and medical sciences
Biotechnology
Dinitrotoluene
Electron Spin Resonance Spectroscopy
Electrons
Free Radicals
Fundamental and applied biological sciences. Psychology
Fungi
Glutathione - metabolism
Manganese
Manganese - metabolism
Methods. Procedures. Technologies
Mineralization
Oxidation
Oxidation-Reduction
Peroxidases - metabolism
Phanerochaete - enzymology
Spin Labels
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Summary:Manganese-dependent peroxidase (MnP) H5 from the white-rot fungus Phanerochaete chrysosporium, in the presence of either Mn(II) (10 mM) or GSH (10 mM). was able to mineralize 14C-U-ring-labeled 2-amino-4,6-dinitrotoluene (2-A-4,6-DNT) up to 29% in 12 days. When both Mn(II) and GSH were present, the mineralization extent reached 82%. On the other hand, no significant mineralization was observed in the absence of both Mn(II) and GSH, suggesting the requirement of a mediator [either Mn(II) or GSH] for the degradation of 2-A-4,6-DNT by MnP. Using electron spin resonance (ESR) techniques, it was found that the glutathionyl free radical (GS*) was produced through the oxidation of GSH by MnP in the presence as well as in the absence of Mn(II). GS* was also generated through the direct oxidation of GSH by Mn(III). Our results strongly suggest the involvement of GS* in the GSH-mediated mineralization of 2-A-4,6-DNT by MnP.
ISSN:0175-7598
1432-0614
DOI:10.1007/s002530000436