Green-fluorescent protein facilitates rapid in vivo detection of genetically transformed plant cells

Early detection of plant transformation events is necessary for the rapid establishment and optimization of plant transformation protocols. We have assessed modified versions of the green fluorescent protein (GFP) from Aequorea victoria as early reporters of plant transformation using a dissecting f...

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Bibliographic Details
Published in:Plant cell reports 1999-05, Vol.18 (9), p.707-714
Main Authors: Elliott, A.R, Campbell, J.A, Dugdale, B, Brettell, R.I.S, Grof, C.P.L
Format: Article
Language:English
Subjects:
Agrobacterium tumefaciens
animal proteins
Bacteria
biochemical techniques
biolistics
Biological and medical sciences
Biotechnology
cell division
detection
Fundamental and applied biological sciences. Psychology
gene expression
Genetic engineering
Genetic technics
genetic transformation
Lactuca sativa
Methods. Procedures. Technologies
Nicotiana tabacum
Plant species
reporter genes
Saccharum officinarum
Scyphozoa
Sugarcane
Transgenic animals and transgenic plants
Transgenic plants
Zea mays
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Summary:Early detection of plant transformation events is necessary for the rapid establishment and optimization of plant transformation protocols. We have assessed modified versions of the green fluorescent protein (GFP) from Aequorea victoria as early reporters of plant transformation using a dissecting fluorescence microscope with appropriate filters. Gfp-expressing cells from four different plant species (sugarcane, maize, lettuce, and tobacco) were readily distinguished, following either Agrobacterium-mediated or particle bombardment-mediated transformation. The identification of gfp-expressing sucarcane cells allowed for the elimination of a high proportion of non-expressing explants and also enabled visual selection of dividing transgenic cells, an early step in the generation of transgenic organisms. The recovery of transgenic cell clusters was streamlined by the ability to visualize gfp-expressing tissues in vitro.
ISSN:0721-7714
1432-203X
DOI:10.1007/s002990050647