Unusual lipid composition of a Bacillus sp. Isolated from Lake Pomorie in Bulgaria

• Published in: Lipids 2000-12, Vol.35 (12), p.1371-n/a
• Main Authors: Carballeira, Nstor M., Guzmn, Aikomari, Nechev, Jordan T., Lahtchev, Kantcho, Ivanova, Albena, Stefanov, Kamen
• Format: Article
• Language: English
• Subjects: Bacillus - chemistry; Bulgaria; Disulfides - chemistry; Fatty acids; Fatty Acids - metabolism; Gas Chromatography-Mass Spectrometry; Hydrocarbons; Hydrocarbons - chemistry; Infrared spectroscopy; Lakes; Linoleic Acids - classification; Linoleic Acids - metabolism; Lipids - chemistry; Spectrophotometry, Infrared
• ISSN: 0024-4201; 1558-9307
• DOI: 10.1007/s11745-000-0654-3

The lipid composition of a Bacillus sp., isolated from Lake Pomorie in Bulgaria, was unusual and consisted of 26 different fatty acids between C12 and C26, with anteiso C15−C17 saturated fatty acids predominating. The furan fatty acid, 10,13‐epoxy‐11‐methyloctadeca‐10,12‐dienoic acid, was also identified, a new finding for this genus. The hydrocarbons consisted of 30 different monounsaturated hydrocarbons, between C25 and C30, with the iso‐iso, iso‐anteiso, anteiso‐anteiso, iso‐normal, and anteiso‐normal methyl branching for odd‐numbered chains, and the iso‐iso, iso‐anteiso, iso‐normal, and anteiso‐normal methyl branching for even‐numbered chains. The double bond positions in these hydrocarbons were determined by dimethyl disulfide derivatization followed by GC‐MS, and the double‐bond cis configuration was confirmed by infrared spectroscopy. Some previously unknown hydrocarbons in bacteria, such as (Z)‐3,21‐dimethyl‐9‐tricosene, (Z)‐3,21‐dimethyl‐10‐tricosene, (Z)‐2,24‐dimethyl‐11‐pentacosene, and (Z)‐2,25‐dimethyl‐13‐hexacosene were identified. Sterols were detected and were based on the sitosterol nucleus.