The important role of glycine N-methyltransferase in the carcinogenesis and progression of prostate cancer

Glycine N-methyltransferase ( GNMT ) has a role in the metabolism of methionine as well as in gluconeogenesis. It has recently been reported that the GNMT gene acts as a tumor-susceptible gene. However, little is known about the specific function of GNMT in carcinogenesis and malignant progression....

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Bibliographic Details
Published in:Modern pathology 2011-09, Vol.24 (9), p.1272-1280
Main Authors: Song, Yoo Hyun, Shiota, Masaki, Kuroiwa, Kentaro, Naito, Seiji, Oda, Yoshinao
Format: Article
Language:English
Subjects:
631/45/612/1238
692/420/755
692/699/67/589/466
Aged
Antibodies
Apoptosis
Biomarkers, Tumor - analysis
Blotting, Western
Cell cycle
Cell Cycle - physiology
Cell growth
Cell Line, Tumor
Cell Proliferation
Cell Separation
Disease Progression
Flow Cytometry
Gene Knockdown Techniques
Glycine N-Methyltransferase - metabolism
Humans
Laboratory Medicine
Liver cancer
Male
Medical prognosis
Medicine
Medicine & Public Health
Metabolism
Metabolites
Middle Aged
Neoplasm Staging
original-article
Pathology
Prognosis
Prostate cancer
Prostatic Neoplasms - enzymology
Prostatic Neoplasms - pathology
Proteins
RNA, Small Interfering
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Summary:Glycine N-methyltransferase ( GNMT ) has a role in the metabolism of methionine as well as in gluconeogenesis. It has recently been reported that the GNMT gene acts as a tumor-susceptible gene. However, little is known about the specific function of GNMT in carcinogenesis and malignant progression. To better our understanding of the function of GNMT in prostate cancer, we used siRNAs to examine the effects of GNMT knockdown on cell proliferation and the cell cycle. In addition, the relation between immunohistochemical GNMT expression and clinicopathologic parameters was investigated in 148 prostate cancer tissues. Here, we show that siRNA-mediated GNMT knockdown results in an inhibition of proliferation, and induces G1 arrest and apoptosis in prostate cancer cell lines. Moreover, high cytoplasmic GNMT expression was also correlated with a higher Gleason score ( P
ISSN:0893-3952
1530-0285
DOI:10.1038/modpathol.2011.76