Molecular cytogenetic characterization of a new wheat Secale africanum 2R^sup a^(2D) substitution line for resistance to stripe rust

A stable, highly fertile wheat Secale africanum substitution line LF24, derived from the F^sub 7^ generation of a cross between Mianyang11 (MY11) and Triticum durum, S. africanum amphiploid (YF) was identified through molecular cytogenetic analysis. Application of C-banding, in situ hybridization an...

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Bibliographic Details
Published in:Journal of genetics 2011-08, Vol.90 (2), p.283
Main Authors: Lei, Mengping, Li, Guangrong, Zhang, Sufen, Liu, Cheng, Yang, Zujun
Format: Article
Language:English
Subjects:
Genetics
Molecular biology
Resistance to control
Wheat
Online Access:Get full text
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Summary:A stable, highly fertile wheat Secale africanum substitution line LF24, derived from the F^sub 7^ generation of a cross between Mianyang11 (MY11) and Triticum durum, S. africanum amphiploid (YF) was identified through molecular cytogenetic analysis. Application of C-banding, in situ hybridization and molecular markers analysis showed that LF24 was a wheat S. africanum 2R^sup a^(2D) substitution line. When inoculated with stripe rust isolates, T. durum and MY11 were highly susceptible, while S. africanum, YF and LF24 were immune. It is confirmed through molecular cytogenetic analysis that the stripe rust resistance of LF24 was derived from S. africanum chromosome 2R^sup a^. We compared the banding patterns and disease resistance of reported chromosomes 2R from different S. cereale introduced into wheat background, and found that there was new stripe rust resistance gene(s) on S. africanum 2R^sup a^. LF24 is a new substitution line which can be used as stripe rust resistant source in wheat improvement.[PUBLICATION ABSTRACT]
ISSN:0022-1333
0973-7731
DOI:10.1007/s12041-011-0081-y