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Suppressive effect of aqueous humor on lipopolysaccharide-induced dendritic cell maturation
Purpose The aqueous humor (AH) contains numerous immunosuppressive molecules that contribute to the ocular immune privilege. Here, we mimic an inflammatory environment to analyze the inhibitory effects of the AH on lipopolysaccharide (LPS)-induced maturation of dendritic cells (DC). Methods Differen...
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| Published in: | Japanese journal of ophthalmology 2011-09, Vol.55 (5), p.558-564 |
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| cites | cdi_FETCH-LOGICAL-c394t-509b76aa0cfd2f7af318c645b8723d41dbbb228ccad66c26203eb9c10e31b3263 |
| container_end_page | 564 |
| container_issue | 5 |
| container_start_page | 558 |
| container_title | Japanese journal of ophthalmology |
| container_volume | 55 |
| creator | Wang, Hui-Fang Liu, Jin-Ling Jiang, Xin-Li Lu, Jian-Min Li, Xiao-Lei Song, Xiu-Jun |
| description | Purpose
The aqueous humor (AH) contains numerous immunosuppressive molecules that contribute to the ocular immune privilege. Here, we mimic an inflammatory environment to analyze the inhibitory effects of the AH on lipopolysaccharide (LPS)-induced maturation of dendritic cells (DC).
Methods
Different concentrations of AH were added to dendritic cell cultures together with LPS. Dendritic cell surface markers CD80, CD86, and MHC-II were assessed by use of flow cytometry. Endocytic capability and mixed lymphocyte reaction were measured as functional maturation.
Results
AH inhibited LPS-induced DC maturation, resulting in down-regulated expression of CD80, CD86, MHC-II, enhancement of endocytic capacity, and reduced T cell activation. Neutralizing transforming growth factor beta 2 (TGF-β
2
) in AH can totally reverse the inhibitory effect. Treatment with prostaglandin E2 (PGE
2
) antagonist alone had no effect on DC maturation. However, blocking of both TGF-β
2
and PGE
2
in the AH resulted in synergistic suppression of the inhibiting effect of AH.
Conclusions
These results reveal that TGF-β
2
in the AH is of crucial importance in maintaining DC in the immature state. Further experiments will clarify the immune role of PGE
2
in AH. |
| doi_str_mv | 10.1007/s10384-011-0060-0 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_895306778</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2473015821</sourcerecordid><originalsourceid>FETCH-LOGICAL-c394t-509b76aa0cfd2f7af318c645b8723d41dbbb228ccad66c26203eb9c10e31b3263</originalsourceid><addsrcrecordid>eNp1kD1PwzAQhi0EoqXwA1iQxW4424mdjKjiS0JiACYGy_EHTZXEwU6Q-u9J1QIT0w33vO-dHoTOKVxRAHmdKPAiI0ApARBA4ADNqaCcMJaJQzQHYJTkNM9n6CSlNQBkjLNjNGNUSi5zmKP3l7Hvo0up_nLYee_MgIPH-nN0YUx4NbYh4tDhpu5DH5pN0sasdKytI3VnR-Mstq6zsR5qg41rGtzqYYx6qEN3io68bpI7288Feru7fV0-kKfn-8flzRMxvMwGkkNZSaE1GG-Zl9pzWhiR5VUhGbcZtVVVMVYYo60QhgkG3FWloeA4rTgTfIEud719DNPfaVDrMMZuOqmKMucgpCwmiO4gE0NK0XnVx7rVcaMoqK1NtbOpJptqa1PBlLnYF49V6-xv4kffBLAdkKZV9-Hi3-X_W78BamuA5A</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>895306778</pqid></control><display><type>article</type><title>Suppressive effect of aqueous humor on lipopolysaccharide-induced dendritic cell maturation</title><source>Springer Link</source><creator>Wang, Hui-Fang ; Liu, Jin-Ling ; Jiang, Xin-Li ; Lu, Jian-Min ; Li, Xiao-Lei ; Song, Xiu-Jun</creator><creatorcontrib>Wang, Hui-Fang ; Liu, Jin-Ling ; Jiang, Xin-Li ; Lu, Jian-Min ; Li, Xiao-Lei ; Song, Xiu-Jun</creatorcontrib><description>Purpose
The aqueous humor (AH) contains numerous immunosuppressive molecules that contribute to the ocular immune privilege. Here, we mimic an inflammatory environment to analyze the inhibitory effects of the AH on lipopolysaccharide (LPS)-induced maturation of dendritic cells (DC).
Methods
Different concentrations of AH were added to dendritic cell cultures together with LPS. Dendritic cell surface markers CD80, CD86, and MHC-II were assessed by use of flow cytometry. Endocytic capability and mixed lymphocyte reaction were measured as functional maturation.
Results
AH inhibited LPS-induced DC maturation, resulting in down-regulated expression of CD80, CD86, MHC-II, enhancement of endocytic capacity, and reduced T cell activation. Neutralizing transforming growth factor beta 2 (TGF-β
2
) in AH can totally reverse the inhibitory effect. Treatment with prostaglandin E2 (PGE
2
) antagonist alone had no effect on DC maturation. However, blocking of both TGF-β
2
and PGE
2
in the AH resulted in synergistic suppression of the inhibiting effect of AH.
Conclusions
These results reveal that TGF-β
2
in the AH is of crucial importance in maintaining DC in the immature state. Further experiments will clarify the immune role of PGE
2
in AH.</description><identifier>ISSN: 0021-5155</identifier><identifier>EISSN: 1613-2246</identifier><identifier>DOI: 10.1007/s10384-011-0060-0</identifier><identifier>PMID: 21773750</identifier><language>eng</language><publisher>Tokyo: Springer Japan</publisher><subject>Animals ; Antibodies, Neutralizing - pharmacology ; Aqueous Humor - physiology ; B7-1 Antigen - metabolism ; B7-2 Antigen - metabolism ; Cells, Cultured ; Dendritic Cells - drug effects ; Dendritic Cells - immunology ; Dextrans - metabolism ; Dinoprostone - antagonists & inhibitors ; Dinoprostone - physiology ; Down-Regulation ; Endocytosis - physiology ; Enzyme-Linked Immunosorbent Assay ; Flow Cytometry ; Fluorescein-5-isothiocyanate - analogs & derivatives ; Fluorescein-5-isothiocyanate - metabolism ; Histocompatibility Antigens Class II - metabolism ; Laboratory Investigation ; Lipopolysaccharides - toxicity ; Lymphocyte Activation ; Lymphocyte Culture Test, Mixed ; Male ; Medicine ; Medicine & Public Health ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Ophthalmology ; Prostaglandin Antagonists - pharmacology ; Swine ; T-Lymphocytes - immunology ; Transforming Growth Factor beta2 - antagonists & inhibitors ; Transforming Growth Factor beta2 - physiology ; Xanthones - pharmacology</subject><ispartof>Japanese journal of ophthalmology, 2011-09, Vol.55 (5), p.558-564</ispartof><rights>Japanese Ophthalmological Society 2011</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c394t-509b76aa0cfd2f7af318c645b8723d41dbbb228ccad66c26203eb9c10e31b3263</citedby><cites>FETCH-LOGICAL-c394t-509b76aa0cfd2f7af318c645b8723d41dbbb228ccad66c26203eb9c10e31b3263</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21773750$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Hui-Fang</creatorcontrib><creatorcontrib>Liu, Jin-Ling</creatorcontrib><creatorcontrib>Jiang, Xin-Li</creatorcontrib><creatorcontrib>Lu, Jian-Min</creatorcontrib><creatorcontrib>Li, Xiao-Lei</creatorcontrib><creatorcontrib>Song, Xiu-Jun</creatorcontrib><title>Suppressive effect of aqueous humor on lipopolysaccharide-induced dendritic cell maturation</title><title>Japanese journal of ophthalmology</title><addtitle>Jpn J Ophthalmol</addtitle><addtitle>Jpn J Ophthalmol</addtitle><description>Purpose
The aqueous humor (AH) contains numerous immunosuppressive molecules that contribute to the ocular immune privilege. Here, we mimic an inflammatory environment to analyze the inhibitory effects of the AH on lipopolysaccharide (LPS)-induced maturation of dendritic cells (DC).
Methods
Different concentrations of AH were added to dendritic cell cultures together with LPS. Dendritic cell surface markers CD80, CD86, and MHC-II were assessed by use of flow cytometry. Endocytic capability and mixed lymphocyte reaction were measured as functional maturation.
Results
AH inhibited LPS-induced DC maturation, resulting in down-regulated expression of CD80, CD86, MHC-II, enhancement of endocytic capacity, and reduced T cell activation. Neutralizing transforming growth factor beta 2 (TGF-β
2
) in AH can totally reverse the inhibitory effect. Treatment with prostaglandin E2 (PGE
2
) antagonist alone had no effect on DC maturation. However, blocking of both TGF-β
2
and PGE
2
in the AH resulted in synergistic suppression of the inhibiting effect of AH.
Conclusions
These results reveal that TGF-β
2
in the AH is of crucial importance in maintaining DC in the immature state. Further experiments will clarify the immune role of PGE
2
in AH.</description><subject>Animals</subject><subject>Antibodies, Neutralizing - pharmacology</subject><subject>Aqueous Humor - physiology</subject><subject>B7-1 Antigen - metabolism</subject><subject>B7-2 Antigen - metabolism</subject><subject>Cells, Cultured</subject><subject>Dendritic Cells - drug effects</subject><subject>Dendritic Cells - immunology</subject><subject>Dextrans - metabolism</subject><subject>Dinoprostone - antagonists & inhibitors</subject><subject>Dinoprostone - physiology</subject><subject>Down-Regulation</subject><subject>Endocytosis - physiology</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Flow Cytometry</subject><subject>Fluorescein-5-isothiocyanate - analogs & derivatives</subject><subject>Fluorescein-5-isothiocyanate - metabolism</subject><subject>Histocompatibility Antigens Class II - metabolism</subject><subject>Laboratory Investigation</subject><subject>Lipopolysaccharides - toxicity</subject><subject>Lymphocyte Activation</subject><subject>Lymphocyte Culture Test, Mixed</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Inbred C57BL</subject><subject>Ophthalmology</subject><subject>Prostaglandin Antagonists - pharmacology</subject><subject>Swine</subject><subject>T-Lymphocytes - immunology</subject><subject>Transforming Growth Factor beta2 - antagonists & inhibitors</subject><subject>Transforming Growth Factor beta2 - physiology</subject><subject>Xanthones - pharmacology</subject><issn>0021-5155</issn><issn>1613-2246</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNp1kD1PwzAQhi0EoqXwA1iQxW4424mdjKjiS0JiACYGy_EHTZXEwU6Q-u9J1QIT0w33vO-dHoTOKVxRAHmdKPAiI0ApARBA4ADNqaCcMJaJQzQHYJTkNM9n6CSlNQBkjLNjNGNUSi5zmKP3l7Hvo0up_nLYee_MgIPH-nN0YUx4NbYh4tDhpu5DH5pN0sasdKytI3VnR-Mstq6zsR5qg41rGtzqYYx6qEN3io68bpI7288Feru7fV0-kKfn-8flzRMxvMwGkkNZSaE1GG-Zl9pzWhiR5VUhGbcZtVVVMVYYo60QhgkG3FWloeA4rTgTfIEud719DNPfaVDrMMZuOqmKMucgpCwmiO4gE0NK0XnVx7rVcaMoqK1NtbOpJptqa1PBlLnYF49V6-xv4kffBLAdkKZV9-Hi3-X_W78BamuA5A</recordid><startdate>20110901</startdate><enddate>20110901</enddate><creator>Wang, Hui-Fang</creator><creator>Liu, Jin-Ling</creator><creator>Jiang, Xin-Li</creator><creator>Lu, Jian-Min</creator><creator>Li, Xiao-Lei</creator><creator>Song, Xiu-Jun</creator><general>Springer Japan</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20110901</creationdate><title>Suppressive effect of aqueous humor on lipopolysaccharide-induced dendritic cell maturation</title><author>Wang, Hui-Fang ; Liu, Jin-Ling ; Jiang, Xin-Li ; Lu, Jian-Min ; Li, Xiao-Lei ; Song, Xiu-Jun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c394t-509b76aa0cfd2f7af318c645b8723d41dbbb228ccad66c26203eb9c10e31b3263</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Antibodies, Neutralizing - pharmacology</topic><topic>Aqueous Humor - physiology</topic><topic>B7-1 Antigen - metabolism</topic><topic>B7-2 Antigen - metabolism</topic><topic>Cells, Cultured</topic><topic>Dendritic Cells - drug effects</topic><topic>Dendritic Cells - immunology</topic><topic>Dextrans - metabolism</topic><topic>Dinoprostone - antagonists & inhibitors</topic><topic>Dinoprostone - physiology</topic><topic>Down-Regulation</topic><topic>Endocytosis - physiology</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Flow Cytometry</topic><topic>Fluorescein-5-isothiocyanate - analogs & derivatives</topic><topic>Fluorescein-5-isothiocyanate - metabolism</topic><topic>Histocompatibility Antigens Class II - metabolism</topic><topic>Laboratory Investigation</topic><topic>Lipopolysaccharides - toxicity</topic><topic>Lymphocyte Activation</topic><topic>Lymphocyte Culture Test, Mixed</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Inbred C57BL</topic><topic>Ophthalmology</topic><topic>Prostaglandin Antagonists - pharmacology</topic><topic>Swine</topic><topic>T-Lymphocytes - immunology</topic><topic>Transforming Growth Factor beta2 - antagonists & inhibitors</topic><topic>Transforming Growth Factor beta2 - physiology</topic><topic>Xanthones - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Hui-Fang</creatorcontrib><creatorcontrib>Liu, Jin-Ling</creatorcontrib><creatorcontrib>Jiang, Xin-Li</creatorcontrib><creatorcontrib>Lu, Jian-Min</creatorcontrib><creatorcontrib>Li, Xiao-Lei</creatorcontrib><creatorcontrib>Song, Xiu-Jun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Proquest Health & Medical Complete</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Japanese journal of ophthalmology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Hui-Fang</au><au>Liu, Jin-Ling</au><au>Jiang, Xin-Li</au><au>Lu, Jian-Min</au><au>Li, Xiao-Lei</au><au>Song, Xiu-Jun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Suppressive effect of aqueous humor on lipopolysaccharide-induced dendritic cell maturation</atitle><jtitle>Japanese journal of ophthalmology</jtitle><stitle>Jpn J Ophthalmol</stitle><addtitle>Jpn J Ophthalmol</addtitle><date>2011-09-01</date><risdate>2011</risdate><volume>55</volume><issue>5</issue><spage>558</spage><epage>564</epage><pages>558-564</pages><issn>0021-5155</issn><eissn>1613-2246</eissn><abstract>Purpose
The aqueous humor (AH) contains numerous immunosuppressive molecules that contribute to the ocular immune privilege. Here, we mimic an inflammatory environment to analyze the inhibitory effects of the AH on lipopolysaccharide (LPS)-induced maturation of dendritic cells (DC).
Methods
Different concentrations of AH were added to dendritic cell cultures together with LPS. Dendritic cell surface markers CD80, CD86, and MHC-II were assessed by use of flow cytometry. Endocytic capability and mixed lymphocyte reaction were measured as functional maturation.
Results
AH inhibited LPS-induced DC maturation, resulting in down-regulated expression of CD80, CD86, MHC-II, enhancement of endocytic capacity, and reduced T cell activation. Neutralizing transforming growth factor beta 2 (TGF-β
2
) in AH can totally reverse the inhibitory effect. Treatment with prostaglandin E2 (PGE
2
) antagonist alone had no effect on DC maturation. However, blocking of both TGF-β
2
and PGE
2
in the AH resulted in synergistic suppression of the inhibiting effect of AH.
Conclusions
These results reveal that TGF-β
2
in the AH is of crucial importance in maintaining DC in the immature state. Further experiments will clarify the immune role of PGE
2
in AH.</abstract><cop>Tokyo</cop><pub>Springer Japan</pub><pmid>21773750</pmid><doi>10.1007/s10384-011-0060-0</doi><tpages>7</tpages></addata></record> |
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| ispartof | Japanese journal of ophthalmology, 2011-09, Vol.55 (5), p.558-564 |
| issn | 0021-5155 1613-2246 |
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| subjects | Animals Antibodies, Neutralizing - pharmacology Aqueous Humor - physiology B7-1 Antigen - metabolism B7-2 Antigen - metabolism Cells, Cultured Dendritic Cells - drug effects Dendritic Cells - immunology Dextrans - metabolism Dinoprostone - antagonists & inhibitors Dinoprostone - physiology Down-Regulation Endocytosis - physiology Enzyme-Linked Immunosorbent Assay Flow Cytometry Fluorescein-5-isothiocyanate - analogs & derivatives Fluorescein-5-isothiocyanate - metabolism Histocompatibility Antigens Class II - metabolism Laboratory Investigation Lipopolysaccharides - toxicity Lymphocyte Activation Lymphocyte Culture Test, Mixed Male Medicine Medicine & Public Health Mice Mice, Inbred BALB C Mice, Inbred C57BL Ophthalmology Prostaglandin Antagonists - pharmacology Swine T-Lymphocytes - immunology Transforming Growth Factor beta2 - antagonists & inhibitors Transforming Growth Factor beta2 - physiology Xanthones - pharmacology |
| title | Suppressive effect of aqueous humor on lipopolysaccharide-induced dendritic cell maturation |
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