Biodegradation of Total Petroleum Hydrocarbons from Oily Sludge

• Published in: Bioremediation journal 2011-07, Vol.15 (3), p.140-147
• Main Authors: Srinivasarao Naik, B., Mishra, I. M., Bhattacharya, S. D.
• Format: Article
• Language: English
• Subjects: Biodegradation; Biodegradation of pollutants; Biological and medical sciences; Bioremediation; Biotechnology; Crude oil; Degradation; Environment and pollution; Fundamental and applied biological sciences. Psychology; Hydrocarbons; Industrial applications and implications. Economical aspects; Kinetics; Microorganisms; oily sludge; Rate constants; Sludge; Slurries; TPH
• ISSN: 1547-6529; 1088-9868; 1547-6529
• DOI: 10.1080/10889868.2011.598484

The present study concerns the biotreatment of oily sludge of petroleum refineries. Experiments were performed to assess the degradation potential of the microbial species present in the oily sludge through augmentation, and using the augmented microbial inoculums to treat oily sludge in a slurry form containing mineral medium and water. The optimum pH of the slurry was found to be 8 for the biodegradation of oily sludge. The effect of oily sludge concentration, stirring rate, and treatment time on degradation of total petroleum hydrocarbons (TPH) was studied. It was found that the degradation process consists of two consecutive stages and that each stage follows a first-order kinetics. The first stage lasted 4 days followed by a second stage up to 7 days. The faster first stage had a rate constant of 0.1 day⁻¹, whereas the slower second stage had a rate constant of 0.056 day⁻¹. The kinetics was found to be time-dependent. The study showed that the mineral medium provided essential nutrients to the microbial species and that the degradation efficiency of the whole microbial species present in the oily sludge was quite high (∼90%).