Identification of Classifiers for Increase or Decrease of Thyroid Peroxidase Activity in the FTC-238/hTPO Recombinant Cell Line

Thyroid peroxidase (TPO) plays an important role in thyroid hormone biosynthesis, as it catalyzes all of the essential steps in iodide organification. TPO activity can be detected using the guaiacol assay; however, this assay is complex and very time-consuming. Therefore, we focused on devising a si...

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Bibliographic Details
Published in:Environmental science & technology 2011-09, Vol.45 (18), p.7906-7914
Main Authors: Song, Mee, Kim, Youn-Jung, Song, Mi-Kyung, Choi, Han-Seam, Park, Yong-Keun, Ryu, Jae-Chun
Format: Article
Language:English
Subjects:
Bioassays
Biological and medical sciences
Biosynthesis
Catalysis
Cell Line, Tumor
Cell Proliferation - drug effects
Chemical and industrial products toxicology. Toxic occupational diseases
Ecotoxicology and Human Environmental Health
Environmental Pollutants - toxicity
Gene expression
Gene Expression Profiling
Gene Expression Regulation, Enzymologic - drug effects
Hormones
Humans
Iodide Peroxidase - genetics
Iodide Peroxidase - metabolism
Medical sciences
Microarray Analysis
Thyroid gland
Toxicology
Transfection
Various organic compounds
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Summary:Thyroid peroxidase (TPO) plays an important role in thyroid hormone biosynthesis, as it catalyzes all of the essential steps in iodide organification. TPO activity can be detected using the guaiacol assay; however, this assay is complex and very time-consuming. Therefore, we focused on devising a simplified method using microarrays to detect changes in TPO activity, which is a target for disruption of the thyroid hormone axis. These experiments have systematically assessed the potential utility of transcriptomic end points as enhancements to the guaiacol assay. Previously, we demonstrated that benzophenone-2, benzophenone, perfluorooctane sulfonate, bisphenol A bis ether, and vinclozolin decreased TPO activity, and that dibutyl phthalate, carbaryl, dibenzo(a,h)anthracene, benzo(a)pyrene, and methylmercury increased TPO activity. In this work, we used human oligonucleotide chips to examine changes in the gene expression profile of FTC-238 human follicular thyroid carcinoma cells expressing human recombinant TPO, after exposure of the cells to TPO activity-disrupting agents. We identified 362 classifiers that could predict the effect of the toxicants on TPO activity with about 70% accuracy. These classifiers are potential markers for predicting the effects of chemicals on thyroid hormone production.
ISSN:0013-936X
1520-5851
DOI:10.1021/es200475k