Photoacoustic Imaging of Multiple Targets Using Gold Nanorods

Photoacoustic (PA) imaging has been used mainly for anatomical and functional imaging. Although functionalized nanoparticles also have been developed for PA molecular imaging, only single targeting has been demonstrated. In this study, PA imaging of multiple targets using gold nanorods is demonstrat...

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Bibliographic Details
Published in:IEEE transactions on ultrasonics, ferroelectrics, and frequency control ferroelectrics, and frequency control, 2007-08, Vol.54 (8), p.1642-1647
Main Authors: Li, P.-C., Wei, C.-W., Liao, C.-K., Chen, C.-D., Pao, K.-C., Wang, C.-R.C., Wu, Y.-N., Shieh, D.-B.
Format: Article
Language:English
Subjects:
Absorption
Animals
Aspect ratio
Biological and medical sciences
Biomedical imaging
Biomedical optical imaging
Blotting, Western
Cancer
Contrast Media
Gold
Gold - chemistry
Humans
Image Enhancement - methods
Imaging
Investigative techniques, diagnostic techniques (general aspects)
Laser-Doppler Flowmetry - instrumentation
Laser-Doppler Flowmetry - methods
Lasers
Liver Neoplasms - diagnostic imaging
Medical sciences
Mice
Miscellaneous. Technology
Nanoparticles
Nanorods
Nanotubes - chemistry
Noise levels
Optical imaging
Optical surface waves
Pathogens
Surface treatment
Tumor Cells, Cultured - diagnostic imaging
Ultrasonic investigative techniques
Ultrasonography
Urinary Bladder Neoplasms - diagnostic imaging
Wavelengths
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Summary:Photoacoustic (PA) imaging has been used mainly for anatomical and functional imaging. Although functionalized nanoparticles also have been developed for PA molecular imaging, only single targeting has been demonstrated. In this study, PA imaging of multiple targets using gold nanorods is demonstrated experimentally using HER2 and CXCR4 as target molecules. The two corresponding monoclonal antibodies were conjugated to two types of gold nanorod with different aspect ratios. Gold nanorods with mean aspect ratios of 5.9 and 3.7 exhibited peak optical absorptions at 1000 and 785 nm, respectively. Appropriate selection of laser irradiation wavelength enhances PA signals by 7-12 dB and allows signals from gold nanorods corresponding to specific bindings to be distinguished. This approach potentially allows the expression levels of different oncogenes of cancer cells to be revealed simultaneously.
ISSN:0885-3010
1525-8955
DOI:10.1109/TUFFC.2007.435