Resonant Cavity Imaging: A Means Toward High-Throughput Label-Free Protein Detection

The resonant cavity imaging biosensor (RCIB) is an optical technique for detecting molecular binding interactions label free at many locations in parallel that employs an optical resonant cavity for high sensitivity. Near-infrared light centered at 1512.5 nm couples resonantly through a Fabry-Perot...

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Bibliographic Details
Published in:IEEE journal of selected topics in quantum electronics 2008-01, Vol.14 (1), p.131-139
Main Authors: Bergstein, David A., Ozkumur, Emre, Wu, Arthur C., Yalcin, Ayca, Colson, Jeremy R., Needham, James W., Irani, Rostem J., Gershoni, Jonathan M., Goldberg, Bennett B., DeLisi, Charles, Ruane, Michael F., Unlu, M. Selim
Format: Article
Language:English
Subjects:
Binding
Biochemistry
Biomedical optical imaging
Biosensors
chemical transducers
Construction
Etching
Holes
Imaging
Optical detectors
Optical imaging
optical resonance
Optical sensors
Optical surface waves
Pixels
Proteins
Resonance
Silicon dioxide
Testing
Wavelengths
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Summary:The resonant cavity imaging biosensor (RCIB) is an optical technique for detecting molecular binding interactions label free at many locations in parallel that employs an optical resonant cavity for high sensitivity. Near-infrared light centered at 1512.5 nm couples resonantly through a Fabry-Perot cavity constructed from dielectric reflectors (Si/SiO 2 ), one of which serves as the binding surface. As the wavelength is swept using a tunable laser, a near-infrared digital camera monitors cavity transmittance at each pixel. A wavelength shift in the local resonant response of the optical cavity indicates binding. Positioning the sensing surface with respect to the standing wave pattern of the electric field within the cavity controls the sensitivity with which the presence of bound molecules is detected. Transmitted intensity at thousands of pixel locations is recorded simultaneously in a 10 s, 5 nm scan. An initial proof-of-principle setup has been constructed. A test sample was fabricated with 25,100-mum wide square features, each with a different density of 1-mum square depressions etched 12 nm into the SiO 2 surface. The average depth of each etched region was found with 0.05 nm rms precision. In a second test, avidin, bound selectively to biotin conjugated bovine serum albumin, was detected.
ISSN:1077-260X
1558-4542
DOI:10.1109/JSTQE.2007.913397