Experience-dependent regulation of CaMKII activity within single visual cortex synapses in vivo

Unbalanced visual input during development induces persistent alterations in the function and structure of visual cortical neurons. The molecular mechanisms that drive activity-dependent changes await direct visualization of underlying signals at individual synapses in vivo. By using a genetically e...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2011-12, Vol.108 (52), p.21241-21246
Main Authors: Mower, Amanda F., Kwok, Showming, Yu, Hongbo, Majewska, Ania K., Okamoto, Ken-Ichi, Hayashi, Yasunori, Sur, Mriganka
Format: Article
Language:English
Subjects:
Animals
Biological Sciences
Ca super(2+)/calmodulin-dependent protein kinase II
Calcium-Calmodulin-Dependent Protein Kinase Type 2 - metabolism
Cell Line
Cortex
Cortex (visual)
Dominance, Ocular - physiology
Enzyme Activation - physiology
Eye
Eyes
Eyes & eyesight
Ferrets
Fluorescence Resonance Energy Transfer
Fluorescent Dyes - metabolism
Genetic engineering
Humans
Image Processing, Computer-Assisted
Imaging
Kinases
Molecular modelling
Monocular vision
Neurochemistry
Neuroimaging
Neurons
Neuroscience
Photic Stimulation
Plasticity
Probes
Pyramidal cells
Sensory Deprivation - physiology
Signal transduction
Structure-function relationships
Synapses
Synapses - enzymology
Synapses - physiology
T tests
Vision, Monocular - physiology
Visual cortex
Visual Cortex - enzymology
Visual Cortex - physiology
Visual deprivation
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Summary:Unbalanced visual input during development induces persistent alterations in the function and structure of visual cortical neurons. The molecular mechanisms that drive activity-dependent changes await direct visualization of underlying signals at individual synapses in vivo. By using a genetically engineered Förster resonance energy transfer (FRET) probe for the detection of CaMKII activity, and two-photon imaging of single synapses within identified functional domains, we have revealed unexpected and differential mechanisms in specific subsets of synapses in vivo. Brief monocular deprivation leads to activation of CaMKII in most synapses of layer 2/3 pyramidal cells within deprived eye domains, despite reduced visual drive, but not in nondeprived eye domains. Synapses that are eliminated in deprived eye domains have low basal CaMKII activity, implying a protective role for activated CaM-KII against synapse elimination.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.1108261109