In vitro culture of Hypericum rumeliacum Boiss. and production of phenolics and flavonoids

The Balkan endemic species, Hypericum rumeliacum, Guttiferae was introduced in vitro for the first time with the aim to study the type of morphogenetic response to plant growth regulators and ability to produce phenolics and flavonoid compounds. The morphoregulatory effect of 2,4-dichlorophenoxyacet...

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Bibliographic Details
Published in:In vitro cellular & developmental biology. Plant 2010-10, Vol.46 (5), p.422-429
Main Authors: Danova, Kalina, Čellárová, Eva, Macková, Anna, Daxnerová, Zuzana, Kapchina-Toteva, Veneta
Format: Article
Language:English
Subjects:
Antimicrobial agents
Antioxidants
Bacteria
Biomedical and Life Sciences
Callus
Cell Biology
Developmental Biology
Endemic species
Flavonoids
Growth regulators
Histology
Internodes
Introduced species
Life Sciences
Metabolites
MORPHOGENESIS
Phenols
Plant Breeding/Biotechnology
Plant cells
Plant Genetics and Genomics
Plant growth
Plant growth regulators
Plant Sciences
Plants
Secondary metabolites
Shoots
Slavic culture
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Summary:The Balkan endemic species, Hypericum rumeliacum, Guttiferae was introduced in vitro for the first time with the aim to study the type of morphogenetic response to plant growth regulators and ability to produce phenolics and flavonoid compounds. The morphoregulatory effect of 2,4-dichlorophenoxyacetic acid (2,4-D), 1-naphtaleneacetic acid (NAA), 6-benzyladenine (BA) and combination of BA with NAA in Murashige-Skoog's basal medium on leaf lamina, internode stem segment, stem node and root cuttings was studied. Histological analysis of the structures regenerated from the primary explants proved the presence of both, embryoids and meristemoids. The node explants cultivated on BA-supplemented medium were the most favourable for regeneration through meristemoids. Therefore a double-stage culture approach, allowing an effective multiplication of large quantities of plant shoots in vitro along with maintenance of the biosynthetic capacity of the culture was developed. It comprised one subculture of three-nodal stem explants derived from the stock shoot cultures on MS medium supplemented with 0.2 mg/l BA followed by subculture of the induced multiple shoots on cytokinin-free MS medium. Determination of the total phenolics and flavonoids showed that the decrease of the levels of these secondary metabolites is transitional, as the exclusionof BA from the medium resulted in an increase of their total content.
ISSN:1054-5476
1475-2689
DOI:10.1007/s11627-010-9299-2