In vitro shoot induction and plant regeneration from flower buds in Paphiopedilum orchids

Paphiopedilum species are recalcitrant in tissue culture, and no explant from mature plants has been successfully mass propagated in vitro. This study was aimed at inducing shoots and regenerating plants from the flowering plants of a sequentially flowering Paphiopedilum Deperle and a single floral...

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Bibliographic Details
Published in:In vitro cellular & developmental biology. Plant 2011-12, Vol.47 (6), p.702-709
Main Authors: Liao, Yu-Ju, Tsai, Yu-Ching, Sun, Yung-Wei, Lin, Ruey-Song, Wu, Fang-Sheng
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Bracts
Buds
Cell Biology
Developmental Biology
Flower buds
flowering
Flowering plants
Flowers
Flowers & plants
greenhouses
Hybridity
Inflorescences
Life Sciences
mature plants
Meristems
Micropropagation
Paphiopedilum
Plant Breeding/Biotechnology
Plant Genetics and Genomics
Plant Sciences
Plant Tissue Culture
Plants
Regeneration
Seeds
Shoots
Slippers
tissue culture
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Summary:Paphiopedilum species are recalcitrant in tissue culture, and no explant from mature plants has been successfully mass propagated in vitro. This study was aimed at inducing shoots and regenerating plants from the flowering plants of a sequentially flowering Paphiopedilum Deperle and a single floral Paphiopedilum Armeni White. By using cross-sectioned flower buds (FBs), we found that in both species, only sections that contained the base tissue of FBs were able to produce shoots and plants. We have also found that sections of FBs between 1.5 and 3.0 cm from Paphiopedilum Deperle were able to produce shoots, but only sections of FBs >2.5 cm from Paphiopedilum Armeni White were regenerable. Our microscopic observations revealed that the small bract at the FB base harbored a new miniature FB, which further harbored a primitive FB with dome-shaped meristem-like tissues that presumably led to the plant induction. The reiteration of this pattern resulted in a scorpioid cyme inflorescence architecture in the multifloral Paphiopedilum species, and its failure to reiterate resulted in a single flower. The induction rates were 57–75%, and all plants survived in a greenhouse. This method is potentially applicable for the micropropagation and conservation of slipper orchids.
ISSN:1054-5476
1475-2689
DOI:10.1007/s11627-011-9370-7