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Protective Effects of Melatonin against Oxidative Stress in Flow Cytometry‐sorted Buffalo Sperm
Previous reports of the ability of melatonin to scavenge a variety of toxic oxygen and nitrogen‐based reactants suggest that melatonin could be an effective antioxidant for protecting sperm. In this study, flow cytometry and laser tweezers Raman spectroscopy were used to evaluate the effect of melat...
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Published in: | Reproduction in domestic animals 2012-04, Vol.47 (2), p.299-307 |
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Main Authors: | , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Previous reports of the ability of melatonin to scavenge a variety of toxic oxygen and nitrogen‐based reactants suggest that melatonin could be an effective antioxidant for protecting sperm. In this study, flow cytometry and laser tweezers Raman spectroscopy were used to evaluate the effect of melatonin on buffalo sperm quality to optimize sperm sex‐sorting procedures. In fresh sperm incubated in the presence or absence of melatonin (10−4 m) for 1, 24, 48 h or 72 h at 27°C, the mitochondrial activity was significantly higher than in a non‐melatonin control (p |
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ISSN: | 0936-6768 1439-0531 |
DOI: | 10.1111/j.1439-0531.2011.01858.x |