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Protective Effects of Melatonin against Oxidative Stress in Flow Cytometry‐sorted Buffalo Sperm

Previous reports of the ability of melatonin to scavenge a variety of toxic oxygen and nitrogen‐based reactants suggest that melatonin could be an effective antioxidant for protecting sperm. In this study, flow cytometry and laser tweezers Raman spectroscopy were used to evaluate the effect of melat...

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Bibliographic Details
Published in:Reproduction in domestic animals 2012-04, Vol.47 (2), p.299-307
Main Authors: Li, XX, Yang, XG, Lu, YQ, Lu, SS, Zhang, M, Yao, HL, Meng, LJ, Lu, KH
Format: Article
Language:English
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Summary:Previous reports of the ability of melatonin to scavenge a variety of toxic oxygen and nitrogen‐based reactants suggest that melatonin could be an effective antioxidant for protecting sperm. In this study, flow cytometry and laser tweezers Raman spectroscopy were used to evaluate the effect of melatonin on buffalo sperm quality to optimize sperm sex‐sorting procedures. In fresh sperm incubated in the presence or absence of melatonin (10−4 m) for 1, 24, 48 h or 72 h at 27°C, the mitochondrial activity was significantly higher than in a non‐melatonin control (p 
ISSN:0936-6768
1439-0531
DOI:10.1111/j.1439-0531.2011.01858.x