Optimization of in vitro micropropagation and regeneration for Populus × interamericana and Populus × euramericana hybrids (P. deltoides, P. trichocarpa, and P. nigra)

A rapid and efficient micropropagation method has been established for six European poplar cultivars of economic interest - four Populus × interamericana and two Populus × euramericana. Using a three-step procedure, we were able to regenerate plantlets from callus and acclimate them within 4 months....

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Bibliographic Details
Published in:Plant cell reports 2002-06, Vol.20 (12), p.1150-1155
Main Authors: Noël, N., Leplé, J.-C., Pilate, G.
Format: Article
Language:English
Subjects:
Cultivars
Genetic engineering
Hybrids
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Summary:A rapid and efficient micropropagation method has been established for six European poplar cultivars of economic interest - four Populus × interamericana and two Populus × euramericana. Using a three-step procedure, we were able to regenerate plantlets from callus and acclimate them within 4 months. In the first step, callogenesis was induced when explants were cultured for 25 days on culture medium supplemented with 10 µM α-naphthaleneacetic acid and 5 µM N^sup 6^(2-isopentenyl)adenine. Bud regeneration followed by shoot elongation was then obtained from callus tissue by combining the cytokinin-like compound thidiazuron with the surfactant Pluronic F-68 at concentrations adjusted for each cultivar. The usefulness of this procedure in the area of genetic engineering is discussed.[PUBLICATION ABSTRACT]
ISSN:0721-7714
1432-203X
DOI:10.1007/s00299-002-0465-9