Optimization of Enzymatic Synthesis of Phytosteryl Caprylates Using Response Surface Methodology

Phytosterols are known to lower total blood cholesterol and low-density lipoprotein (LDL) cholesterol. However, low solubility of phytosterols in edible oils and their high melting point limit their use in food products. Esterification of phytosterols with fatty acids will render them higher solubil...

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Bibliographic Details
Published in:Journal of the American Oil Chemists' Society 2012-04, Vol.89 (4), p.657-666
Main Authors: Tan, Zhuliang, Shahidi, Fereidoon
Format: Article
Language:English
Subjects:
Agriculture
antimicrobial properties
Bioconversions. Hemisynthesis
Biological and medical sciences
Biomaterials
Biotechnology
blood
candidiasis
Chemical synthesis
Chemistry
Chemistry and Materials Science
Cholesterol
Edible oils
energy
Energy sources
Enzymes
Esterification
Fat industries
Food industries
Food Science
foods
Fundamental and applied biological sciences. Psychology
Industrial Chemistry/Chemical Engineering
Lipoproteins
low density lipoprotein
Melting
Melting point
Methods. Procedures. Technologies
nutritionists
octanoic acid
oils
Original Paper
Phosphate esters
Phytochemicals
Phytosterols
Phytosteryl caprylates
response surface methodology
Response surface methodology (RSM)
Solubility
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Summary:Phytosterols are known to lower total blood cholesterol and low-density lipoprotein (LDL) cholesterol. However, low solubility of phytosterols in edible oils and their high melting point limit their use in food products. Esterification of phytosterols with fatty acids will render them higher solubility in oils and lowers their melting points. In this work, caprylic acid (C8:0) was selected for the esterification process because it serves as a rapid energy source with little or no deposition in the body and is often recommended by nutritionists for the treatment of candidiasis due to its antimicrobial properties. Here optimization of enzymatic synthesis of phytosteryl caprylates using response surface methodology (RSM) was investigated. The optimization process used a face-centred central composite design (CCD) and under optimized conditions (9.25 h, 2.15 substrate mole ratio, and 7.93% enzyme load) the resultant conversion yield was 98%.
ISSN:0003-021X
1558-9331
DOI:10.1007/s11746-011-1949-y