Temporal expression analysis and cloning of cotton (Gossypium hirsutum) fiber genes

Cotton fiber, a seed trichome and the longest plant cell, is an important model system to dissect the molecular genetic basis of fiber cell development processes. The expression patterns of 19 fiber specific genes in cotton (Gossypium hirsutum L.) were determined by RT-PCR and Northern blots. Total...

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Bibliographic Details
Published in:International journal of agriculture and biology 2011-01, Vol.13 (1), p.89-94
Main Authors: Indrais, E. (National Inst. for Biotechnology and Genetic Engineering, Faisalabad (Pakistan)), Cheema, H.M.N. (University of Agriculture, Faisalabad (Pakistan). Dept. of Plant Breeding and Genetics), Samad, A. (National Inst. for Biotechnology and Genetic Engineering, Faisalabad (Pakistan)), Bashir, A. (University of Agriculture, Faisalabad (Pakistan). Dept. of Plant Breeding and Genetics)
Format: Article
Language:English
Subjects:
FIBRES
GENE EXPRESSION
GOSSYPIUM HIRSUTUM
MOLECULAR CLONING
NUCLEOTIDE SEQUENCE
PCR
RNA
VARIETIES
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Summary:Cotton fiber, a seed trichome and the longest plant cell, is an important model system to dissect the molecular genetic basis of fiber cell development processes. The expression patterns of 19 fiber specific genes in cotton (Gossypium hirsutum L.) were determined by RT-PCR and Northern blots. Total RNA, extracted from cotton fibers at 0, 2, 5, 10, 15 and 20 days post anthesis (DPA), was reverse transcribed to cDNA for PCR amplification and cloning. Transcripts of expansion and tubulin were observed from 5 to 15 DPA. Lipid transfer protein (LTP) and E6 gene transcription was evident throughout fiber development. Out of the ten partially characterized genes, five showed expression patterns similar to LTP and E6, while the remainder showed distinct profiles. This information is valuable in selecting promoters for the fiber specific expression of transgenes in cotton species.
ISSN:1560-8530
1814-9596