Effect of Vitrification of Feline Ovarian Cortex on Follicular and Oocyte Quality and Competence

Cryopreservation of ovarian cortex has important implications in the preservation of fertility and biodiversity in animal species. Slow freezing of cat ovarian tissue resulted in the preservation of follicular morphology and in the follicular development after xenografting. Vitrification has been re...

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Bibliographic Details
Published in:Reproduction in domestic animals 2012-06, Vol.47 (3), p.385-391
Main Authors: Luvoni, GC, Tessaro, I, Apparício, M, Ruggeri, E, Luciano, AM, Modina, SC
Format: Article
Language:English
Subjects:
actin
Animal sciences
Animals
biodiversity
Biological and medical sciences
Cats
Cats - physiology
chromatin
cortex
cryopreservation
Cryopreservation - veterinary
Cryotherapy
Female
Females
follicular development
freezing
Fundamental and applied biological sciences. Psychology
germinal vesicle
Histology
Mammalian reproduction. General aspects
meiosis
microfilaments
microtubules
Oocytes - cytology
Oocytes - physiology
Ovarian Follicle - physiology
ovarian follicles
Reproductive system
Vertebrates: reproduction
vitrification
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Summary:Cryopreservation of ovarian cortex has important implications in the preservation of fertility and biodiversity in animal species. Slow freezing of cat ovarian tissue resulted in the preservation of follicular morphology and in the follicular development after xenografting. Vitrification has been recently applied to ovarian tissues of different species, but no information is available on the effect of this method on feline ovarian cortex. Moreover, meiotic competence of fully grown oocytes isolated from cryopreserved tissue has not been reported. The aim of this study was to evaluate the effect of vitrification of feline ovarian cortex on follicular morphology and oocyte integrity, as well as meiotic competence. A total of 352 fragments (1.5–2 mm3) were obtained from ovarian cortical tissues: 176 were vitrified and 176 were used fresh as control. Histological evaluation of fresh and vitrified fragments showed intact follicles after cryopreservation procedures with no statistically significant destructive effect from primordial to antral follicles. After IVM, oocytes collected from vitrified ovarian fragment showed a higher proportion of gametes arrested at germinal vesicle (GV) stage compared to those isolated from fresh control tissue (33.8% vs 2.9%; p 
ISSN:0936-6768
1439-0531
DOI:10.1111/j.1439-0531.2011.01885.x