Chlorpyrifos modifies the expression of genes involved in human placental function

• Published in: Reproductive toxicology (Elmsford, N.Y.) N.Y.), 2012-06, Vol.33 (3), p.331-338
• Main Authors: Ridano, Magali E., Racca, Ana C., Flores-Martín, Jésica, Camolotto, Soledad A., de Potas, Gladis Magnarelli, Genti-Raimondi, Susana, Panzetta-Dutari, Graciela M.
• Format: Article
• Language: English
• Subjects: ATP Binding Cassette Transporter, Sub-Family G, Member 2; ATP-Binding Cassette Transporters - genetics; Biological and medical sciences; Cell Line, Tumor; Cell Survival - drug effects; Chlorpyrifos - toxicity; Chorionic Gonadotropin, beta Subunit, Human - genetics; Embryology: invertebrates and vertebrates. Teratology; Female; Fundamental and applied biological sciences. Psychology; Gene expression; Gene Expression Regulation, Developmental - drug effects; Humans; Insecticides - toxicity; Medical sciences; Neoplasm Proteins - genetics; Nuclear Proteins - genetics; Organophosphate pesticide; Pesticides, fertilizers and other agrochemicals toxicology; Placenta - drug effects; Placenta - metabolism; Pregnancy; RNA, Messenger - metabolism; Teratology. Teratogens; Toxicology; Transcription Factors - genetics; Trophoblast; βhCG
• ISSN: 0890-6238; 1873-1708
• DOI: 10.1016/j.reprotox.2012.01.003

► Organophosphate pesticides are suspected to increase the risk of placental dysfunction. ► We analyzed the effect of chlorpyrifos (CPF) on a trophoblast in vitro cell model. ► CPF alters the expression of βhCG, GCM1 and ABCG2 genes. ► βhCG transcript, protein and secretion are markedly augmented in cells exposed to CPF. ► These genes may represent novel targets of in vivo exposure of placenta to CPF. The effects of organophosphate pesticides on human placenta remain poorly investigated although an increased risk of pregnancy alterations has been reported in women chronically exposed to these pesticides. Here, we have addressed whether chlorpyrifos (CPF) modifies the expression of genes relevant for placental function. Human placental JEG-3 cells were exposed to increasing CPF concentrations up to 100μM for 24 and 48h and cell viability, mRNA, protein and hormone levels were analyzed. Quantitative RT-PCR assays revealed that CPF increased the expression of ABCG2, GCM1 and, even more significantly, βhCG mRNAs in conditions where cell viability and morphology were not compromised. In addition, βhCG protein synthesis and secretion were time-dependently augmented. Present results may reflect a CPF nocive effect on placenta cells or a placental-defense mechanism to preserve its function. These novel CPF trophoblast target genes should be considered in future studies of pregnancy outcomes associated with in vivo exposures.